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Expression of the thymosin beta 4 gene during differentiation of
hematopoietic cells
R Shimamura, J Kudo, H Kondo, K Dohmen, H Gondo, S Okamura, H Ishibashi and Y Niho
First Department of Internal Medicine, Faculty of Medicine, Kyushu
University, Fukuoka, Japan.
Thymosin beta 4 (T beta 4) was originally isolated as a thymic hormone. Its
functional properties remain obscure; however, the N-terminal peptidic
sequence could have a regulatory function on hematopoietic stem cell
proliferation. To investigate the mechanism of T beta 4 expression, we
studied T beta 4 gene expression in various leukemic cells and in
established cell lines. Among leukemic cell samples obtained from leukemia
patients, the T beta 4 gene was highly expressed in a lymphoid lineage,
especially in adult T-cell leukemia (ATL) cells, rather than in a
granulocyte lineage. The T beta 4 gene was more transcriptionally active in
chronic B-cell leukemia than in acute B- cell leukemia, while it was
inactive in plasma cell leukemia. We also found that cells from one of the
ATL patients transcribed a heterogeneous message. T beta 4 messenger RNA
increased in MOLT-3 during differentiation by
12-O-tetradecanoylphorbol-13-acetate (TPA), in HL60 cells induced by TPA or
dimethylsulfoxide and K562 cells stimulated by cytosine arabinoside or
hemin. The genomic sequence of T beta 4 is considered to be highly
conserved. Only 1 of 20 genomes from normal or hematopoietic malignant
cells showed restriction fragment length polymorphism. These findings,
along with previous data, suggest that T beta 4 may be a new marker of
differentiation of hematopoietic cells.
Volume 76,
Issue 5,
pp. 977-984,
09/01/1990
Copyright © 1990 by The American Society of Hematology
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