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Murine leukemia inhibitory factor enhances retroviral-vector infection
efficiency of hematopoietic progenitors
FA Fletcher, DE Williams, C Maliszewski, D Anderson, M Rives and JW Belmont
Howard Hughes Medical Institute, Houston, TX.
We have investigated the in vitro effects of the cytokine leukemia
inhibitory factor (LIF) on normal murine hematopoietic progenitors by
measuring recovery and retroviral vector infection efficiency of 13-day
posttransplant, spleen-colony-forming cell (CFU-S 13) in short-term
culture. Up to a twofold increase in CFU-S13 recovery was observed, from
9.7 x 10(-5) cells in untreated controls to 17.8 to 19.5 x 10(-5) cells,
depending on the concentration of LIF. Histologic analysis of spleen
colonies from control and LIF-treated marrows demonstrated that there was
no detectable alteration in the differentiative potential of CFU-S13. The
efficiency of CFU-S13 infection was increased from 15% in untreated
controls to 84% to 91% in LIF-treated marrows. Analysis of proviral
integration sites in spleen colonies indicated that some CFU- S13
precursors were infected in the LIF-treated marrows.
Volume 76,
Issue 6,
pp. 1098-1103,
09/15/1990
Copyright © 1990 by The American Society of Hematology
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