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Identification of hematopoietic progenitors of macrophages and dendritic
Langerhans cells (DL-CFU) in human bone marrow and peripheral blood
CD Reid, PR Fryer, C Clifford, A Kirk, J Tikerpae and SC Knight
Section of Haematology, Clinical Research Centre, Harrow, UK.
Colonies of cells with distinctive dendritic appearance were observed in
methylcellulose cultures of human bone marrow and peripheral blood
mononuclear cells (PBMC). Such cells appeared alone in colonies of less
than 50 cells, together with macrophages in mixed colonies and also within
clusters of T lymphocytes at high culture cell numbers. The morphologic
resemblance to lymphoid dendritic cells was confirmed by electron
microscopy and the cells were distinguished from macrophages by
immunoenzymatic and immunogold labeling with monoclonal antibodies (MoAbs).
Like macrophages they were HLA-DR+ and CD4+. However, they lacked
nonspecific esterase and the macrophage cytoplasmic marker Y1/82A. Most
strikingly, cells were strongly HLA-DQ+ and expressed CD1a (T6), which is
characteristic of skin Langerhans cells. Their functional similarity to
lymphoid dendritic cells was demonstrated by their ability to stimulate
allogeneic mixed leukocyte reactions. Dendritic cell colony numbers were
estimated in both bone marrow and peripheral blood of controls and in
leukemia and lymphoma patients before and after chemotherapy. Colony
numbers were low in control blood and in patients before treatment (less
than 1.0 to 3.7/10(5) cells). However, during hematopoietic recovery the
mean value increased to 37.5/10(5) cells and this increase correlated
closely with the observed increase in circulating colony forming
unit-granulocyte macrophage (CFU- GM) in individual patients.
Autoradiographic studies demonstrated mitotic activity within CD1a+
colonies and a linear relationship between cultured cells and both pure and
mixed colonies was consistent with their derivation from a single
precursor. These data indicate that a novel hematopoietic progenitor of
dendritic/Langerhans cells (DL-CFU) may now be identified in a clonal assay
system and suggest a probable common progenitor for these cells and
macrophages.
Volume 76,
Issue 6,
pp. 1139-1149,
09/15/1990
Copyright © 1990 by The American Society of Hematology
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