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This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Strair, R. K. Articles by Smith, B. R. Search for Related Content PubMed PubMed Citation Articles by Strair, R. K. Articles by Smith, B. R. Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  Retroviral mediated transfer and expression of exogenous genes in primary lymphoid cells: assaying for a viral transactivator activity in normal and malignant cells RK Strair, M Towle, PW Heald and BR Smith Department of Internal Medicine, Yale University School of Medicine, New Haven, CT 06510. In this report we describe the use of recombinant retroviruses to characterize the activity of an exogenous promoter in primary cells obtained from patients with lymphoproliferative disorders. The infection of a variety of cultured and primary lymphoid cells with a recombinant retrovirus containing a histone promoter-driven beta- galactosidase gene is shown to result in the expression of beta- galactosidase in 50% to 100% of the cells. A similar infection with a recombinant retrovirus containing the beta-galactosidase gene with an adenovirus E2 promoter, results in beta-galactosidase activity in a limited number of cultured and primary cells. Since the adenovirus E2 promoter has been well characterized and is known to be regulated by transactivators encoded by many viruses, the activity of this promoter in specific cell types is discussed in reference to both the biology of the cell and the possible presence of as yet undetected viral gene products. Volume 76, Issue 6, pp. 1201-1208, 09/15/1990 Copyright © 1990 by The American Society of Hematology CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: S.-H. Fang, B.-L. Chiang, M.-H. Wu, H. Iba, M.-Y. Lai, P.-M. Yang, D.-S. Chen, and L.-H. Hwang Functional Measurement of Hepatitis C Virus Core-Specific CD8+ T-Cell Responses in the Livers or Peripheral Blood of Patients by Using Autologous Peripheral Blood Mononuclear Cells as Targets or Stimulators J. Clin. Microbiol., November 1, 2001; 39(11): 3895 - 3901. [Abstract] [Full Text] [PDF]

	Copyright © 1990 by American Society of Hematology         Online ISSN: 1528-0020