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CD34+ hematopoietic progenitor cells are not a major reservoir of the human
immunodeficiency virus
D von Laer, FT Hufert, TE Fenner, S Schwander, M Dietrich, H Schmitz and P Kern
Clinical and Virological Department, Bernhard-Nocht Institute for Tropical
Medicine, Hamburg, FRG.
Hematologic abnormalities occur in the majority of patients with acquired
immunodeficiency syndrome (AIDS). Infection of the hematopoietic progenitor
cells has been proposed as a potential explanation. In this study,
different bone marrow cell populations, including the CD34+ hematopoietic
progenitor cells, were purified by a fluorescence-activated cell sorter
(FACS) and analyzed for the presence of human immunodeficiency virus-1
(HIV-1) proviral DNA using the polymerase chain reaction. A group of 14
patients with AIDS or AIDS- related complex (ARC) was studied (11 with
peripheral blood cytopenias). The CD4+ helper cells in the bone marrow were
found positive for HIV-1 DNA in all patients. In contrast, CD34+ progenitor
cells were positive in only one patient. Two monocyte samples and two
samples of CD4-/CD34- lymphocytes/blasts (mainly B and CD8 lymphocytes)
were positive. Proviral DNA could not be detected in granulocytes. FACS
analysis showed that the percentage of CD34+ hematopoietic progenitor cells
was not altered in the bone marrow of AIDS patients in comparison with the
HIV-1 seronegative controls. In contrast, the number of CD4+ lymphocytes
was markedly reduced in the bone marrow of AIDS patients. These results
show that the hematologic abnormalities in AIDS patients are neither
explained by direct infection of the hematopoietic progenitor cells with
HIV-1 nor by a depletion of progenitor cells.
Volume 76,
Issue 7,
pp. 1281-1286,
10/01/1990
Copyright © 1990 by The American Society of Hematology
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