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Induction of mixed erythroid-megakaryocyte colonies and bipotential blast
cell colonies by recombinant human erythropoietin in serum-free culture
N Nishi, T Nakahata, K Koike, M Takagi, K Naganuma and T Akabane
Department of Pediatrics, Shinshu University School of Medicine, Matsumoto,
Japan.
The effects of recombinant human erythropoietin (rEp) on murine
hematopoietic progenitors were studied using a serum-free culture. A high
concentration of rEp stimulated the formation of mixed erythroid-
megakaryocyte colonies (EM colonies) and blast cell colonies, as well as
erythroid colonies, erythroid bursts, and megakaryocyte colonies from
normal mouse bone marrow cells. Direct effects of rEp on EM colony,
megakaryocyte colony, and erythroid burst formation were confirmed by
depletion of accessory cells such as T cells, B cells, and macrophages from
crude bone marrow cells, and inhibition of the colonies by the addition of
rabbit anti-rEp antibody to the culture in a dose-dependent fashion.
Replating experiments were performed to confirm the differentiating ability
of blast cell colonies grown in the presence of rEp. Most of the blast cell
colonies yielded not only secondary erythroid colonies but also
megakaryocyte colonies in the presence of 2 IU/mL rEp. Some of the blast
cell colonies produced secondary EM colonies in the presence of 16 IU/ml
rEp of 2 IU/mL rEp plus interleukin-3, although no granulocyte-macrophage
colonies were found in the secondary culture. These results suggest that Ep
acts not only as a late-acting factor that is specific for erythroid
progenitors, but also as a bipotential EM-stimulating factor for murine
hematopoietic cells.
Volume 76,
Issue 7,
pp. 1330-1335,
10/01/1990
Copyright © 1990 by The American Society of Hematology
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