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Characterization of a factor-dependent acute leukemia cell line with
translocation (3;3)(q21;q26)
J Oval, OW Jones, M Montoya and R Taetle
Department of Medicine, University of California, San Diego.
A strictly factor-dependent cell line (UCSD/AML1) was established from a
patient with the syndrome of multilineage acute leukemia with high
platelets. The patient's cells and the cell line karyotype were 45,XX,-
7,t(3;3)(q21;q26), typical of the syndrome of acute leukemia with high
platelets. The cell line expresses CD34, CD7, TdT, and myeloid (CD13, CD14,
CD33) and megakaryocyte/platelet (CD36, CD41, CD42b, CDw49b) antigens. In
short-term culture, UCSD/AML1 cells proliferate in response to
interleukin-3 (IL-3), IL-4, IL-6, macrophage colony- stimulating factor
(M-CSF), and granulocyte-macrophage CSF (GM-CSF), but not IL-1, IL-2, IL-5,
or G-CSF. In long-term culture, proliferation can be sustained by GM-CSF,
IL-6, or M-CSF. When maintained in GM-CSF, a small percentage of cells form
multinucleated megakaryocyte-like giant cells. Culture with GM-CSF combined
with IL-6, but not with IL-6 alone, increased giant cell formation fourfold
to sevenfold. IL-6 alone or in combination with GM-CSF increased expression
of platelet-related antigens. In contrast, culture with phorbol ester
induced formation of macrophage-like cells. UCSD/AML1 is the first human
acute nonlymphocytic leukemia cell line established from a patient with an
acute leukemia syndrome associated with a specific chromosome abnormality.
Volume 76,
Issue 7,
pp. 1369-1374,
10/01/1990
Copyright © 1990 by The American Society of Hematology
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