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This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Tweardy, D. J. Articles by Signorella, A. P. Search for Related Content PubMed PubMed Citation Articles by Tweardy, D. J. Articles by Signorella, A. P. Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  Production of laminin B2 chain protein and messenger RNA by a murine neutrophil precursor cell line, 32Dc13 [see comments] DJ Tweardy, M Sasaki, JJ Cardamone , JP McCoy , MJ Bonidie and AP Signorella Department of Medicine, University of Pittsburgh School of Medicine, PA. Laminin is a heterotrimeric glycoprotein that plays a central role in promoting neutrophil chemotaxis, motility, and attachment to basement membrane. Rabbit peritoneal exudate neutrophils stain positively for laminin, which is presumed to be of exogenous origin and bound to laminin receptors on the cell surface. We examined 32Dc13 cells, a murine neutrophil precursor cell line, by immunoprecipitation. Northern blot analysis, flow cytometry, and electron microscopy for the endogenous production of laminin. Our results demonstrate that 32Dc13 cells endogenously produce a laminin B2 chain protein and messenger RNA (mRNA) without producing any detectable A or B1 chain protein or mRNA. The B2 chain protein was not secreted by the cells; rather it could be detected on the cell surface after treatment of cells with neuraminidase. These findings suggest the possibility of a novel role for the laminin B2 chain in myeloid development and function. Volume 76, Issue 7, pp. 1398-1404, 10/01/1990 Copyright © 1990 by The American Society of Hematology CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: C. Pedraza, T. Geberhiwot, S. Ingerpuu, D. Assefa, Z. Wondimu, J. Kortesmaa, K. Tryggvason, I. Virtanen, and M. Patarroyo Monocytic Cells Synthesize, Adhere to, and Migrate on Laminin-8 ({alpha}4{beta}1{gamma}1) J. Immunol., November 15, 2000; 165(10): 5831 - 5838. [Abstract] [Full Text] [PDF]

	Copyright © 1990 by American Society of Hematology         Online ISSN: 1528-0020