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The role of cellular maturation in neutrophil heterogeneity
PJ Krause, MB Todd, WW Hancock, WT Pastuszak, EG Maderazo, DH Hild and CM Kosciol
Department of Pediatrics, Hartford Hospital, CT 06115.
Previous studies have shown that many neutrophil (PMN) characteristics are
heterogeneous but the origin of PMN heterogeneity is unknown. It is unclear
if PMN functional heterogeneity is secondary to maturational differences or
due to distinct subpopulations of cells that possess different functional
capacities. The PMN 31D8 antigen is a useful probe for evaluation of PMN
subpopulations. The majority of PMNs (approximately 85%) exhibit a high
intensity fluorescence after 31D8 monoclonal antibody (MoAb) labeling (31D8
enriched or "bright" PMNs) as determined by flow cytometric analysis. These
cells are more functional than cells with low intensity fluorescence (31D8
diminished or "dull" PMNs). Various immunologic, clonogenic and functional
techniques were used to study the expression of the 31D8 antigen in HL-60
cells and myeloid cells in order to evaluate antigenic and functional
heterogeneity during morphologic maturation. The results of this study
indicate that the percentage of 31D8 antigen positive (31D8 antigen
enriched and diminished) bone marrow cells increases from 20 +/- 11% in
myeloblast cells to 68 +/- 10% in promyelocytes, 93 +/- 2% in myelocytes
and 99 +/- 1% in bands and PMNs. 31D8 antigen enriched cells first appear
at the myelocyte stage (32 +/- 10%) and increase in bands (52 +/- 13%),
marrow PMNs (62 +/- 13%) and peripheral blood PMNs (88 +/- 4%). These data
indicate that the heterogeneous expression of 31D8 antigen in PMNs is due,
at least in part, to maturational differences within the PMN population and
raise the possibility that other heterogeneously expressed PMN
characteristics are also maturationally derived. They also suggest that
31D8 antigenic expression may be a more precise indicator of myeloid
functional maturation than maturation as identified by cellular morphology.
Volume 76,
Issue 8,
pp. 1639-1646,
10/15/1990
Copyright © 1990 by The American Society of Hematology
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