Developmental regulation of granulocytic cell binding to hemonectin
AD Campbell, MW Long and MS Wicha
Department of Internal Medicine, University of Michigan Medical Center, Ann
Arbor 48105.
Hemonectin (HN), a component of the bone marrow (BM) extracellular matrix
which promotes adhesion of cells in the granulocytic lineage, was purified
to near homogeneity and tested for its ability to mediate attachment of
normal and leukemic cells of granulocytic lineage. Purified HN immobilized
on plastic substrates promoted serum-free attachment of normal
granulocyte/macrophage progenitor cells (CFC-GM), using an in situ
attachment assay in which cell attachment is inhibited by specific
polyclonal antisera. When unfractionated BM cells were allowed to attach to
purified HN and stained in situ, HN preferentially bound cells at earlier
stages of granulocytic differentiation. These observations were confirmed
using cells of the HL-60 progranulocytic cell line which mirrored this
differentiation-stage specific binding to HN. HN promoted attachment of 60%
of uninduced HL-60 cells which were arrested at the progranulocyte stage,
whereas only 15% of uninduced HL- 60 cells attached to uncoated plastic and
4% to attached plastic coated with equal microgram quantities of bovine
serum albumin (BSA). When HL- 60 cells were induced to differentiate along
the granulocytic pathway by incubation with dimethylsulfoxide (DMSO),
attachment to hemonectin was reduced. Thus, both primary BM granulocytic
cells and a granulocytic cell line show preferential attachment of those
cells at earlier stages of differentiation. This developmentally regulated
binding suggests a mechanism for release of maturing BM into the peripheral
circulation.
Volume 76,
Issue 9,
pp. 1758-1764,
11/01/1990
Copyright © 1990 by The American Society of Hematology
