Human macrophage colony-stimulating factor induces macrophage colonies
after L-phenylalanine methylester treatment of human marrow
CS Rosenfeld, C Evans and RK Shadduck
Department of Internal Medicine, Montefiore Hospital, University of
Pittsburgh School of Medicine, PA.
Macrophage-colony stimulating factor (M-CSF) has well-known effects on
murine bone marrow, but its colony stimulating activity for human bone
marrow is controversial. After treatment of human bone marrow with L-
phenylalanine methylester (PME), macrophage-colonies (CFU-M) were induced
by M-CSF in a dose-dependent fashion. The optimal concentration of
recombinant human-macrophage colony stimulating factor (rhM-CSF) was 1,000
U/mL. Purified human urine M-CSF had colony stimulating activity similar to
rhM-CSF. Further studies were performed to determine the factors
responsible for the enhanced CFU-M formation from PME treated marrow.
Compared with nylon wool and carbonyl iron monocyte depletion methods, PME
eliminated significantly more monocytes and myeloid cells. This observation
suggested that these cells may release hematopoietic inhibitory factors for
CFU-M. Low concentrations (1%) but not normal (10%) concentrations of blood
monocytes were inhibitory (mean inhibition, 48%) to CFU-M. High
concentrations of monocytes (50%) augmented CFU-M colonies. HL-60
conditioned media was used to simulate secretory products of early myeloid
cells. HL-60 conditioned media (1%) inhibited CFU-M formation but not
granulocyte macrophage or granulocyte colonies. We conclude that M-CSF has
colony stimulating activity for human marrow that can be recognized after
removal of inhibitory cells by PME treatment.
Volume 76,
Issue 9,
pp. 1783-1787,
11/01/1990
Copyright © 1990 by The American Society of Hematology
