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In vitro rosetting, cytoadherence, and microagglutination properties of
Plasmodium falciparum-infected erythrocytes from Gambian and Tanzanian
patients
T Hasler, SM Handunnetti, JC Aguiar, MR van Schravendijk, BM Greenwood, G Lallinger, P Cegielski and RJ Howard
Laboratory of Infectious Diseases, DNAX Research Institute, Palo Alto, CA
94304-1104.
To understand the molecular mechanisms that lead to sequestration of red
blood cells infected with mature stages of Plasmodium falciparum and to
examine the relevance of earlier studies on adherence properties of
laboratory-derived P falciparum parasites to the natural parasite
population, we analyzed Gambian and Tanzanian isolates for in vitro
cytoadherence and antibody-mediated microagglutination. Eighteen
cryopreserved isolates of ring-stage parasites were cultured for 20 to 30
hours in vitro, in the patients original erythrocytes, to the trophozoite
and schizont stage. All parasites were positive in the microagglutination
assay with at least one of four African hyperimmune sera. In a rosetting
assay, only 2 of the 18 isolates were strongly positive (35% and 41% of
parasitized erythrocytes with more than two uninfected cells bound).
Thirteen isolates showed either intermediate (5% to 18%) or low (less than
5%) rosetting while three isolates did not form rosettes. Infected
cell-binding of the different isolates to immobilized CD36 or
thrombospondin, or C32 melanoma cells correlated with the percentage of
mature parasites in the blood samples (r = .932 for CD36, r = .946 for
thrombospondin, and r = .881 for C32 melanoma cells). There was a high
correlation between binding to CD36 and thrombospondin (r = .982). The
extent of infected cell rosetting with uninfected cells in these blood
samples was not correlated with these other receptor properties. We also
observed coexpression of rosetting and cytoadherence receptors on the same
parasitized erythrocytes.
Volume 76,
Issue 9,
pp. 1845-1852,
11/01/1990
Copyright © 1990 by The American Society of Hematology
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