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Expression of c-myb and B-myb, but not A-myb, correlates with proliferation
in human hematopoietic cells
J Golay, A Capucci, M Arsura, M Castellano, V Rizzo and M Introna
Istituto Ricerche Farmacologiche Mario Negri, Milano, Italy.
The steady-state expression of three members of the myb family of genes,
c-myb, B-myb, and A-myb, was studied in four purified normal human
hematopoietic cell types, ie, T and B lymphocytes, monocytes, and
granulocytes. The c-myb proto-oncogene is low to undetectable in resting T
and B lymphocytes and shows a biphasic induction in both cell types after
mitogenic stimulation, with a first peak at 3 hours and a second and
stronger induction at 44 to 72 hours. Study of the B-myb gene showed that
this gene is low to undetectable in resting T or B cells and is strongly
induced after mitogenic stimulation with a peak between 44 and 72 hours in
both cell types. Finally, the A-myb gene shows a pattern of expression in
lymphocytes different from that of c- myb and B-myb. It is expressed in
resting T lymphocytes and its levels gradually decrease after mitogenic
stimulation to become undetectable at 48 hours. It is also expressed in a
subpopulation of large B lymphocytes but not in in vitro activated B cells.
Neither of the three members of the myb family of genes was expressed in
monocytes and granulocytes, even after functional activation of these
cells. Taken together, these data bring further evidence for the role of
c-myb in cellular proliferation and on the basis of the kinetics of its
induction relative to thymidine uptake, we hypothesize that it may have a
role during G1 progression in addition to that already documented for entry
into S phase. Furthermore, our studies indicate that another member of the
myb family of genes, B-myb, may also be involved in cellular proliferation,
because its expression correlates with the induction of mitogenesis.
Volume 77,
Issue 1,
pp. 149-158,
01/01/1991
Copyright © 1991 by The American Society of Hematology
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