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H Kodama, M Iizuka, T Tomiyama, K Yoshida, M Seki, T Suda and S Nishikawa
Department of Anatomy, Ohu University School of Dentistry, Koriyama, Japan.
Some mouse myeloid leukemias induced by X-irradiation and serially
transplanted into syngenic mice do not proliferate in vitro even in the
presence of hematopoietic factors. To examine whether such leukemic cells
can proliferate in response to stromal cells, we cocultured them with
MC3T3-G2/PA6 (PA6) preadipocytes, cells that can support the growth of
hematopoietic stem cells. All leukemias developed into in vitro cell lines,
showing a dependence on contact with the PA6 cells. Two cell lines
responded to none of the known hematopoietic factors including
interleukin-3 (IL-3), IL-4, IL-5, IL-6, GM-CSF, G-CSF, M-CSF, and Epo.
These results demonstrate that the mechanism of the action of PA6 cells is
different from that of any of the known hematopoietic factors, and that,
because these two leukemic cell lines retained the ability to grow in vivo,
responsiveness to the known hematopoietic factors is not essential for the
leukemic cell growth in vivo. Furthermore, all leukemic cell lines could
respond also to the preadipocytes fixed with formalin, paraformaldehyde, or
glutaraldehyde, suggesting that some molecule(s) associated with the
surface of PA6 cells or with extracellular matrix secreted by the
preadipocytes is responsible for the leukemic cell growth.
This article has been cited by other articles:
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| Copyright © 1991 by American Society of Hematology Online ISSN: 1528-0020 | |||||||||
