|
|
 Previous Article | Table of Contents | Next Article 
FDP D-dimer induces the secretion of interleukin-1, urokinase-type
plasminogen activator, and plasminogen activator inhibitor-2 in a human
promonocytic leukemia cell line
M Hamaguchi, Y Morishita, I Takahashi, M Ogura, J Takamatsu and H Saito
First Department of Internal Medicine, Nagoya University School of
Medicine, Japan.
We studied the effect of fibrinogen degradation products D, E, and D- dimer
on a human promonocytic leukemia cell line, NOMO-1. After exposure to a
10(-5)-mol/L fragment D or D-dimer, the cells displayed macrophage-like
characteristics, such as adherence to plastic surfaces, and showed
approximately a twofold increase in response to the nitroblue tetrazolium
reduction test. The secretion of interleukin-1 alpha (IL-1 alpha) into the
medium was markedly stimulated by a 10(-5)- mol/L fragment D, E, and
D-dimer, whereas a significant increase in IL- 1 beta secretion was
observed only in D-dimer-stimulated cells. In addition, D-dimer induced a
rapid increase in urokinase-type plasminogen activator on day 1 (0.52 +/-
0.02 ng/mL v 0.07 +/- 0.01 ng/mL in the control culture) and a slow
increase in plasminogen activator inhibitor-2 on day 5 (3.9 +/- 1.6 ng/mL v
1.2 +/- 0.2 ng/mL in the control culture). An increase in tissue factor
(TF) was also demonstrated on the cell surface of NOMO-1 cells exposed to
fragment D or D-dimer by indirect immunofluorescence using an anti-TF
monoclonal antibody. Scatchard plot analysis showed that fragment D and
D-dimer bound to the NOMO-1 cells with a kd of 3.3 nmol/L and 2.7 nmol/L,
respectively. These results suggest that fragment D-dimer specifically
stimulates cells of monocyte-macrophage lineage to secrete key substances
that regulate blood coagulation, fibrinolysis, and inflammation.
Volume 77,
Issue 1,
pp. 94-100,
01/01/1991
Copyright © 1991 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
H. Fujimoto, E. C. Gabazza, O. Taguchi, Y. Nishii, H. Nakahara, N. E. Bruno, C. N. D'Alessandro-Gabazza, M. Kasper, Y. Yano, M. Nagashima, et al.
Thrombin-Activatable Fibrinolysis Inhibitor Deficiency Attenuates Bleomycin-Induced Lung Fibrosis
Am. J. Pathol.,
April 1, 2006;
168(4):
1086 - 1096.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. F. Pieper, K. M. K. Rao, M. S. Currie, T. B. Harris, and H. J. Cohen
Age, Functional Status, and Racial Differences in Plasma D-Dimer Levels in Community-Dwelling Elderly Persons
J. Gerontol. A Biol. Sci. Med. Sci.,
November 1, 2000;
55(11):
649M - 657.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
Y. Wakita, H. Wada, T. Nakase, T. Nakasaki, M. Shimura, K. Hiyoyama, Y. Mori, E. C. Gabazza, M. Nishikawa, K. Deguchi, et al.
Aberrations of the Tissue Factor Pathway in Patients Positive for Lupus Anticoagulant
Clinical and Applied Thrombosis/Hemostasis,
January 1, 1999;
5(1):
10 - 15.
[Abstract]
[PDF]
|
|
|
|
|
|
|
D. J. Schneider, M. A. Ricci, D. J. Taatjes, P. Q. Baumann, J. C. Reese, B. J. Leavitt, P. M. Absher, and B. E. Sobel
Changes in Arterial Expression of Fibrinolytic System Proteins in Atherogenesis
Arterioscler. Thromb. Vasc. Biol.,
November 1, 1997;
17(11):
3294 - 3301.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
T. Padro, J. J. Emeis, M. Steins, K. W. Schmid, and J. Kienast
Quantification of Plasminogen Activators and Their Inhibitors in the Aortic Vessel Wall in Relation to the Presence and Severity of Atherosclerotic Disease
Arterioscler. Thromb. Vasc. Biol.,
July 1, 1995;
15(7):
893 - 902.
[Abstract]
[Full Text]
|
|
|
|
|
