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Synergistic effect of recombinant interferon-gamma and interleukin-3 on the
growth of immature human hematopoietic progenitors
Y Kawano, Y Takaue, A Hirao, T Abe, S Saito, K Matsunaga, T Watanabe, M Hirose, T Ninomiya and Y Kuroda
Department of Pediatrics, University Hospital, Tokushima, Japan.
Purified peripheral blood hematopoietic progenitors from children in early
remission from cancer respond to recombinant human interleukin-3 (IL-3),
but not to granulocyte colony-stimulating factor (G-CSF). With these
purified cells as a target, we studied the effect of recombinant human
interferon-gamma (IFN-gamma) on progenitor growth, using both
liquid-suspension limiting dilution assay (LDA) and regular methylcellulose
culture of progenitors. We found that in LDA with IL-3, IFN-gamma directly
stimulated the growth of blood progenitors in a dose- dependent manner with
single-hit kinetics, whereas IFN-gamma suppressed the growth of
G-CSF-supported progenitors obtained from bone marrow. The stimulatory
effect was also observed in methylcellulose culture, but the addition of
antibodies for G-CSF, granulocyte-macrophage CSF, IL-1 alpha, IL-1 beta,
IL-6, or tumor necrosis factor did not result in a decrease of the colony
number, supporting further the possible direct effect of IFN-gamma on
progenitor growth. These results suggest that the inhibitory effect of
IFN-gamma on hematopoietic progenitors is limited to those in an advanced
stage of maturation. IFN-gamma may be one of the essential lymphokines
upregulating the growth of human hematopoietic progenitor cells.
Volume 77,
Issue 10,
pp. 2118-2121,
05/15/1991
Copyright © 1991 by The American Society of Hematology
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