|
|
 Previous Article | Table of Contents | Next Article 
Purification and partial characterization of a human hematopoietic
precursor population
C Smith, C Gasparetto, N Collins, A Gillio, MO Muench, RJ O'Reilly and MA Moore
Bone Marrow Transplant Service, Memorial Sloan Kettering Cancer Center, New
York, NY 10021.
This study reports the development of an assay, the Pre-colony-forming unit
(CFU) assay, which detects human hematopoietic precursors. The Pre- CFU
assay is based on the observation that precursors to CFU-
granulocyte-macrophage (CFU-GM) that are undetectable in clonogenic assays
differentiate into CFU-GM preferentially following treatment in suspension
culture with recombinant human interleukin-1 alpha (rhIL-1 alpha) combined
with rhIL-3. Using the Pre-CFU assay, hematopoietic precursors were
detected in human bone marrow depleted of CFU-GM progenitors and
differentiated hematopoietic elements via 4- hydroperoxycyclophosphamide
treatment coupled with selection for CD34+ cells (4-HCresistant/CD34+
marrow). Additionally, the Pre-CFU assay detected recovery of hematopoiesis
substantially earlier than the CFU- GM assay in primates following
myeloablation with 5-fluorouracil. The Pre-CFU assay was used to asses
purification of a phenotypically defined hematopoietic precursor
population, the lin-CD34+ population. The lin-CD34+ population lacks
detectable surface markers for T-cell, B- cell, natural killer cell, and
myeloid lineage, possesses the CD34 antigen, is devoid of CFU-GM
progenitors, and yields Pre-CFU assay values comparable with
4-HCresistant/CD34+ marrow. Using a combination of phenotypic analysis and
Pre-CFU assay analysis, the action of rhIL-1 alpha plus rhIL-3 treatment on
lin-CD34+ cells was further characterized. The data indicate that rhIL-1
alpha plus rhIL-3 treatment induces proliferation and differentiation of
early hematopoietic precursors into progenitors and terminally
differentiated cells, without inducing a significant expansion of the
precursor population itself.
Volume 77,
Issue 10,
pp. 2122-2128,
05/15/1991
Copyright © 1991 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
T. J. Povsic and P. J. Goldschmidt-Clermont
Review: Endothelial progenitor cells: markers of vascular reparative capacity
Therapeutic Advances in Cardiovascular Disease,
June 1, 2008;
2(3):
199 - 213.
[Abstract]
[PDF]
|
|
|
|
|
|
|
R. W. Storms, A. P. Trujillo, J. B. Springer, L. Shah, O. M. Colvin, S. M. Ludeman, and C. Smith
Isolation of primitive human hematopoietic progenitors on the basis of aldehyde dehydrogenase activity
PNAS,
August 3, 1999;
96(16):
9118 - 9123.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. R. Koller, I. Manchel, and A. K. Smith
Quantitative Long-Term Culture-Initiating Cell Assays Require Accessory Cell Depletion That Can Be Achieved by CD34-Enrichment or 5-Fluorouracil Exposure
Blood,
June 1, 1998;
91(11):
4056 - 4064.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Freedman, D. Neuberg, P. Mauch, J. Gribben, R. Soiffer, K. Anderson, M. Robertson, D. C. Fisher, R. Schlossman, M. Kroon, et al.
Cyclophosphamide, Doxorubicin, Vincristine, Prednisone Dose Intensification With Granulocyte Colony-Stimulating Factor Markedly Depletes Stem Cell Reserve for Autologous Bone Marrow Transplantation
Blood,
December 15, 1997;
90(12):
4996 - 5001.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Berardi, A Wang, J. Levine, P Lopez, and D. Scadden
Functional isolation and characterization of human hematopoietic stem cells
Science,
January 6, 1995;
267(5194):
104 - 108.
[Abstract]
[PDF]
|
|
|
|
|
