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The sequence gamma-(312-324) is a fibrin-specific epitope

WJ Schielen, HP Adams, K van Leuven, M Voskuilen, GI Tesser and W Nieuwenhuizen

Gaubius Institute TNO, Leiden, The Netherlands.

Fibrin accelerates the activation of plasminogen catalyzed by tissue- type plasminogen activator much stronger than fibrinogen. Detailed studies showed that (part of) this rate-enhancing effect of fibrin is brought about by two sites in the fibrin molecule: one in A alpha-(148- 160) and one in the gamma-chain stretch 311-379 (also known as FCB-5). During the fibrinogen-to-fibrin conversion, A alpha-(148-160) appears to become accessible, because a monoclonal antibody against synthetic A alpha-(148-160) reacts with fibrin, but not with fibrinogen. Because a similar situation may exist for (at least parts of) FCB-5, we have prepared a monoclonal antibody against a part (ie, gamma-[312-324]) of FCB-5, and found that this is fibrin-specific and does not bind fibrinogen. We conclude that gamma-(312-324) is hidden in fibrinogen and is exposed by the formation of fibrin.

Volume 77, Issue 10, pp. 2169-2173, 05/15/1991
Copyright © 1991 by The American Society of Hematology


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  Copyright © 1991 by American Society of Hematology         Online ISSN: 1528-0020