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Human herpesvirus-6 in human lymphomas: identification of specific
sequences in Hodgkin's lymphomas by polymerase chain reaction
G Torelli, R Marasca, M Luppi, L Selleri, S Ferrari, F Narni, MT Mariano, M Federico, L Ceccherini-Nelli and M Bendinelli
Center for Experimental Hematology, Second Medical Clinic, Modena, Italy.
In search of a possible involvement of the human herpesvirus type 6 (HHV-6)
in human Hodgkin's and non-Hodgkin's lymphomas, we studied the levels of
anti-HHV-6 antibodies in the sera of 94 cases by an immunofluorescence
assay, as well as the presence of HHV-6 sequences in the affected tissues
of 66 cases by polymerase chain reaction, using one set of primer
oligonucleotides. Our results showed higher anti-HHV- 6 antibody titers in
human lymphomas than in normal blood donors, but the difference is
statistically significant only when normal donors are compared with
Hodgkin's lymphoma cases. HHV-6 sequences were detected in 3 of 25
Hodgkin's lymphomas and 0 of the 41 cases of non-Hodgkin's lymphomas
studied. The three cases positive for HHV-6 sequences belong to the nodular
sclerosis-lymphocyte depletion histologic subtype and share remarkable
similarities in their clinical features. Furthermore, Southern blot
analysis of total genomic DNA obtained from the neoplastic tissues of two
of the three patients showed the same restriction fragment length
polymorphism. Our results suggest that: (1) the high level of anti-HHV-6
antibodies in Hodgkin's disease is due to an activation of the immune
system not related to the presence of HHV-6 sequences in affected lymph
nodes; (2) the presence of HHV-6 sequences in human lymphoid tissues is not
a frequent event, rather it is in fact a very rare event in non-Hodgkin's
lymphomas, while in Hodgkin's cases it is more frequent than previously
reported on the basis of Southern blot analysis; and (3) the presence of
HHV-6 sequences in Hodgkin's lymphomas may have a relation with the
clinical presentation of the disease.
Volume 77,
Issue 10,
pp. 2251-2258,
05/15/1991
Copyright © 1991 by The American Society of Hematology
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