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Recombinant human stem cell factor enhances the formation of colonies by
CD34+ and CD34+lin- cells, and the generation of colony-forming cell
progeny from CD34+lin- cells cultured with interleukin-3, granulocyte
colony-stimulating factor, or granulocyte-macrophage colony-stimulating
factor
ID Bernstein, RG Andrews and KM Zsebo
Program in Pediatric Oncology, Fred Hutchinson Cancer Research Center,
Seattle, WA 98104.
We tested the ability of recombinant human stem cell factor (SCF) to
stimulate isolated marrow precursor cells to form colonies in semisolid
media and to generate colony-forming cells (CFC) in liquid culture. SCF, in
combination with interleukin-3 (IL-3), granulocyte-macrophage
colony-stimulating factor (GM-CSF), or granulocyte colony-stimulating
factor (G-CSF) caused CD34+ cells to form increased numbers of
granulocyte-macrophage colonies (CFU-GM), and to form macroscopic erythroid
burst-forming units (BFU-E) in the presence of IL-3, erythropoietin (Epo),
and SCF. We tested isolated CD34+lin- cells, a minor subset of CD34+ cells
that did not display antigens associated with lymphoid or myeloid lineages,
and CD34+lin+ cells, which contain the vast majority of CFC, and found that
the enhanced colony growth was most dramatic within the CD34+lin-
population. CD34+lin- cells cultured in liquid medium containing SCF
combined with IL-3, GM-CSF, or G-CSF gave rise to increased numbers of CFC.
Maximal numbers of CFU-GM were generated from CD34+lin- cells after 7 to 21
days of culture, and required the presence of SCF from the initiation of
liquid culture. The addition of SCF to IL-3 and/or G-CSF in cultures of
single CD34+lin- cells resulted in increased numbers of CFC due to the
proliferation of otherwise quiescent precursors and an increase in the
numbers of CFC generated from individual precursors. These studies
demonstrate the potent synergistic interaction between SCF and other
hematopoietic growth factors on a highly immature population of CD34+lin-
precursor cells.
Volume 77,
Issue 11,
pp. 2316-2321,
06/01/1991
Copyright © 1991 by The American Society of Hematology
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