|
|
 Previous Article | Table of Contents | Next Article 
Tumor necrosis factor-beta in the serum of adult T-cell leukemia with
hypercalcemia
K Ishibashi, K Ishitsuka, Y Chuman, M Otsuka, Y Kuwazuru, M Iwahashi, A Utsunomiya, S Hanada, T Sakurami and T Arima
Second Department of Internal Medicine, Faculty of Medicine, Kagoshima
University, Japan.
Serum tumor necrosis factor-beta (TNF-beta) from patients with adult T-
cell leukemia (ATL) was studied by a sandwich enzyme-linked immunosorbent
assay (ELISA) developed in our laboratory using biotinylated monoclonal
anti-TNF-beta and recombinant TNF-beta. Seven of eight patients with
hypercalcemia showed elevation of serum TNF- beta. On the other hand,
TNF-beta could not be detected by the ELISA in 28 patients without
hypercalcemia. The lower detection limit in this assay was 100 pg/mL,
corresponding to 500 pg/mL by the conventional method. In two patients
serum TNF-beta level decreased after treatment in association with the
level of serum calcium. Furthermore, immuno- staining using anti-TNF-beta
and avidin-biotin complex showed the presence of cytoplasmic TNF-beta in
not only human T-cell leukemia virus type I infected cell lines, but also
freshly isolated cells from ATL patients with hypercalcemia. The actual
biologic activity of TNF- beta in serum was confirmed by a conventional
bioassay in a patient with hypercalcemia, and its cytotoxic activity was
inhibited by the addition of anti-TNF-beta antibody in the assay. These
results suggested that serum TNF-beta might be one of the factors
contributing to the hypercalcemia, at least in patients with ATL.
Volume 77,
Issue 11,
pp. 2451-2455,
06/01/1991
Copyright © 1991 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
L. Gao, H. Deng, H. Zhao, A. Hirbe, J. Harding, L. Ratner, and K. Weilbaecher
HTLV-1 Tax transgenic mice develop spontaneous osteolytic bone metastases prevented by osteoclast inhibition
Blood,
December 15, 2005;
106(13):
4294 - 4302.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
V. Richard, M. D. Lairmore, P. L. Green, G. Feuer, R. S. Erbe, B. Albrecht, C. D'Souza, E. T. Keller, J. Dai, and T. J. Rosol
Humoral Hypercalcemia of Malignancy : Severe Combined Immunodeficient/Beige Mouse Model of Adult T-Cell Lymphoma Independent of Human T-Cell Lymphotropic Virus Type-1 Tax Expression
Am. J. Pathol.,
June 1, 2001;
158(6):
2219 - 2228.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Mori, M. Fujii, S. Ikeda, Y. Yamada, M. Tomonaga, D. W. Ballard, and N. Yamamoto
Constitutive Activation of NF-kappa B in Primary Adult T-Cell Leukemia Cells
Blood,
April 1, 1999;
93(7):
2360 - 2368.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. L. Kiselev, O. S. Kustikova, E. V. Korobko, E. B. Prokhortchouk, A. A. Kabishev, E. M. Lukanidin, and G. P. Georgiev
Molecular Cloning and Characterization of the Mouse tag7 Gene Encoding a Novel Cytokine
J. Biol. Chem.,
July 17, 1998;
273(29):
18633 - 18639.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
