|
|
 Previous Article | Table of Contents | Next Article 
Multimerin: a series of large disulfide-linked multimeric proteins within
platelets
CP Hayward, TE Warkentin, P Horsewood and JG Kelton
Department of Medicine, McMaster University Medical Centre, Hamilton,
Ontario, Canada.
Platelets contain proteins with biochemical properties that are well
adapted to promoting hemostasis. One important adhesive protein is von
Willebrand factor (vWF), which is a very large protein comprised of a
series of multimers, ranging from 860,000 to over 10 million daltons. In
this report we describe a second platelet protein, p-155, which has a
similar unique multimeric composition. Using agarose-acrylamide gel
electrophoresis, platelet p-155 was shown to be composed of multimers
ranging from less than 450 Kd to many million daltons. Based on this unique
structure, we propose that the native molecule be designated as multimerin.
Comparison with vWF showed that multimerin contained less of the very high
molecular weight multimers. Differential reduction demonstrated that the
smallest multimer is a trimer, composed of three 155-Kd subunits. Platelet
releasate was demonstrated to contain mainly the smaller multimers,
suggesting that the larger multimers bind to the platelet surface. Other
studies indicate that multimerin and vWF are the two largest platelet
proteins and the only two platelet proteins exhibiting a complex,
disulfide-linked multimeric composition with variability in multimer size.
Volume 77,
Issue 12,
pp. 2556-2560,
06/15/1991
Copyright © 1991 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
U. Lewandrowski, R. P. Zahedi, J. Moebius, U. Walter, and A. Sickmann
Enhanced N-Glycosylation Site Analysis of Sialoglycopeptides by Strong Cation Exchange Prefractionation Applied to Platelet Plasma Membranes
Mol. Cell. Proteomics,
November 1, 2007;
6(11):
1933 - 1941.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
U. Lewandrowski, J. Moebius, U. Walter, and A. Sickmann
Elucidation of N-Glycosylation Sites on Human Platelet Proteins: A Glycoproteomic Approach
Mol. Cell. Proteomics,
February 1, 2006;
5(2):
226 - 233.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. G. Mann and M. Kalafatis
Factor V: a combination of Dr Jekyll and Mr Hyde
Blood,
January 1, 2003;
101(1):
20 - 30.
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Christian, H. Ahorn, M. Novatchkova, P. Garin-Chesa, J. E. Park, G. Weber, F. Eisenhaber, W. J. Rettig, and M. C. Lenter
Molecular Cloning and Characterization of EndoGlyx-1, an EMILIN-like Multisubunit Glycoprotein of Vascular Endothelium
J. Biol. Chem.,
December 14, 2001;
276(51):
48588 - 48595.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. P.M. Hayward, Z. Song, S. Zheng, R. Fung, M. Pai, J.-M. Masse, and E. M. Cramer
Multimerin Processing by Cells With and Without Pathways for Regulated Protein Secretion
Blood,
August 15, 1999;
94(4):
1337 - 1347.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Doliana, M. Mongiat, F. Bucciotti, E. Giacomello, R. Deutzmann, D. Volpin, G. M. Bressan, and A. Colombatti
EMILIN, a Component of the Elastic Fiber and a New Member of the C1q/Tumor Necrosis Factor Superfamily of Proteins
J. Biol. Chem.,
June 11, 1999;
274(24):
16773 - 16781.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. P. M. Hayward, E. M. Cramer, Z. Song, S. Zheng, R. Fung, J.-M. Masse, R. H. Stead, and T. J. Podor
Studies of Multimerin in Human Endothelial Cells
Blood,
February 15, 1998;
91(4):
1304 - 1317.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. P.M. Hayward, E. M. Cramer, W. H. Kane, S. Zheng, M. Bouchard, J.-M. Masse, and G. E. Rivard
Studies of a Second Family With the Quebec Platelet Disorder: Evidence That the Degradation of the alpha -Granule Membrane and Its Soluble Contents Are Not Secondary to a Defect in Targeting Proteins to alpha -Granules
Blood,
February 15, 1997;
89(4):
1243 - 1253.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. P. M. Hayward, E. Furmaniak-Kazmierczak, A.-M. Cieutat, J. C. Moore, D. F. Bainton, M. E. Nesheim, J. G. Kelton, and G. Côté
Factor V Is Complexed with Multimerin in Resting Platelet Lysates and Colocalizes with Multimerin in Platelet alpha-Granules
J. Biol. Chem.,
August 18, 1995;
270(33):
19217 - 19224.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. P. M. Hayward, J. A. Hassell, G. A. Denomme, R. A. Rachubinski, C. Brown, and J. G. Kelton
The cDNA Sequence of Human Endothelial Cell Multimerin
J. Biol. Chem.,
August 4, 1995;
270(31):
18246 - 18251.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Mongiat, G. Mungiguerra, S. Bot, M. T. Mucignat, E. Giacomello, R. Doliana, and A. Colombatti
Self-assembly and Supramolecular Organization of EMILIN
J. Biol. Chem.,
August 11, 2000;
275(33):
25471 - 25480.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Doliana, S. Bot, G. Mungiguerra, A. Canton, S. P. Cilli, and A. Colombatti
Isolation and Characterization of EMILIN-2, a New Component of the Growing EMILINs Family and a Member of the EMI Domain-containing Superfamily
J. Biol. Chem.,
April 6, 2001;
276(15):
12003 - 12011.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
