|
|
 Previous Article | Table of Contents | Next Article 
Erythropoietin receptor characteristics on primary human erythroid cells
VC Broudy, N Lin, M Brice, B Nakamoto and T Papayannopoulou
Department of Medicine, University of Washington, Seattle 98195.
Erythropoietin (EP) exerts its effects on erythropoiesis by binding to a
cell surface receptor. We examined EP receptor expression during normal
human erythroid differentiation and maturation from the burst- forming
unit-erythroid (BFU-E) to the reticulocyte level. In contrast to previous
studies, we assessed EP receptor number and affinity in erythroid
precursors immunologically purified from fresh bone marrow aspirates or
fetal liver samples and in reticulocytes purified from peripheral blood. EP
receptors were quantitated by equilibrium binding experiments with 125I EP.
We found that purified primary erythroblasts from both adult and fetal
sources exhibited a single high-affinity (kd 100 pmol/L) binding site for
EP under our experimental conditions, and 135 or 250 receptors per cell,
respectively. Reticulocytes were devoid of EP receptors. We compared these
data to in vitro-derived BFU-E progeny at both early and late stages of
maturation. Cultured BFU-E progeny also displayed a single class of
receptors of slightly lower affinity (210 to 220 pmol/L). Preparations
enriched in colony-forming units-erythroid (CFU-E) and proerythroblasts
(day 9 BFU-E progeny) displayed approximately 1,100 receptors per cell,
whereas populations containing mature erythroblasts (day 14 BFU-E progeny)
exhibited approximately 300 receptors per cell. Furthermore, information
from binding experiments was complemented by autoradiography in both
enriched BFU-E preparations, cultured BFU-E progeny (days 9 and 14), and
marrow mononuclear cells. These studies are consistent with a peak in EP
receptor expression at the CFU-E/proerythroblast stage and a decrease with
further maturation to undetectable levels at the reticulocyte stage. These
data examining EP receptor characteristics on freshly isolated erythroid
precursor cells complement previous data on EP receptor biology using
culture-derived erythroblasts.
Volume 77,
Issue 12,
pp. 2583-2590,
06/15/1991
Copyright © 1991 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
S. Palani and C. A. Sarkar
Positive Receptor Feedback during Lineage Commitment Can Generate Ultrasensitivity to Ligand and Confer Robustness to a Bistable Switch
Biophys. J.,
August 15, 2008;
95(4):
1575 - 1589.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. M. Kirschner, P. Hagen, C. S. Hussels, M. Ballmaier, H. Scholz, and C. Dame
The Wilms' tumor suppressor Wt1 activates transcription of the erythropoietin receptor in hematopoietic progenitor cells
FASEB J,
August 1, 2008;
22(8):
2690 - 2701.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Gutierrez, S. Tsukamoto, M. Suzuki, H. Yamamoto-Mukai, M. Yamamoto, S. Philipsen, and K. Ohneda
Ablation of Gata1 in adult mice results in aplastic crisis, revealing its essential role in steady-state and stress erythropoiesis
Blood,
April 15, 2008;
111(8):
4375 - 4385.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Cazzola
Somatic mutations of JAK2 exon 12 as a molecular basis of erythrocytosis
Haematologica,
December 1, 2007;
92(12):
1585 - 1589.
[Full Text]
[PDF]
|
|
|
|
|
|
|
I. Amoyal, E. Prus, and E. Fibach
Vanadate Elevates Fetal Hemoglobin in Human Erythroid Precursors by Inhibiting Cell Maturation
Experimental Biology and Medicine,
May 1, 2007;
232(5):
654 - 661.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Suzuki, K. Ohneda, S. Hosoya-Ohmura, S. Tsukamoto, O. Ohneda, S. Philipsen, and M. Yamamoto
Real-time monitoring of stress erythropoiesis in vivo using Gata1 and beta-globin LCR luciferase transgenic mice
Blood,
July 15, 2006;
108(2):
726 - 733.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Liu, R. Pop, C. Sadegh, C. Brugnara, V. H. Haase, and M. Socolovsky
Suppression of Fas-FasL coexpression by erythropoietin mediates erythroblast expansion during the erythropoietic stress response in vivo
Blood,
July 1, 2006;
108(1):
123 - 133.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Walrafen, F. Verdier, Z. Kadri, S. Chretien, C. Lacombe, and P. Mayeux
Both proteasomes and lysosomes degrade the activated erythropoietin receptor
Blood,
January 15, 2005;
105(2):
600 - 608.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K.-H. Chang, M. Tam, and M. M. Stevenson
Inappropriately low reticulocytosis in severe malarial anemia correlates with suppression in the development of late erythroid precursors
Blood,
May 15, 2004;
103(10):
3727 - 3735.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. J. Weiss
New Insights Into Erythropoietin and Epoetin Alfa: Mechanisms of Action, Target Tissues, and Clinical Applications
Oncologist,
December 1, 2003;
8(90003):
18 - 29.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Zhang, M. Socolovsky, A. W. Gross, and H. F. Lodish
Role of Ras signaling in erythroid differentiation of mouse fetal liver cells: functional analysis by a flow cytometry-based novel culture system
Blood,
December 1, 2003;
102(12):
3938 - 3946.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Myssina, S. M. Huber, C. Birka, P. A. Lang, K. S. Lang, B. Friedrich, T. Risler, T. Wieder, and F. Lang
Inhibition of Erythrocyte Cation Channels by Erythropoietin
J. Am. Soc. Nephrol.,
November 1, 2003;
14(11):
2750 - 2757.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Li, C. Miller, S. Hegde, and D. Wojchowski
Roles for an Epo Receptor Tyr-343 Stat5 Pathway in Proliferative Co-signaling with Kit
J. Biol. Chem.,
October 17, 2003;
278(42):
40702 - 40709.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Yasuda, Y. Fujita, T. Matsuo, S. Koinuma, S. Hara, A. Tazaki, M. Onozaki, M. Hashimoto, T. Musha, K. Ogawa, et al.
Erythropoietin regulates tumour growth of human malignancies
Carcinogenesis,
June 1, 2003;
24(6):
1021 - 1029.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Suzuki, O. Ohneda, S. Takahashi, M. Higuchi, H. Y. Mukai, T. Nakahata, S. Imagawa, and M. Yamamoto
Erythroid-specific expression of the erythropoietin receptor rescued its null mutant mice from lethality
Blood,
September 18, 2002;
100(7):
2279 - 2288.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Socolovsky, H.-s. Nam, M. D. Fleming, V. H. Haase, C. Brugnara, and H. F. Lodish
Ineffective erythropoiesis in Stat5a-/-5b-/- mice due to decreased survival of early erythroblasts
Blood,
December 1, 2001;
98(12):
3261 - 3273.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
X. Yu, C.-S. Lin, F. Costantini, and C. T. Noguchi
The human erythropoietin receptor gene rescues erythropoiesis and developmental defects in the erythropoietin receptor null mouse
Blood,
July 15, 2001;
98(2):
475 - 477.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. A. Middleton, F. P. Barbone, D. L. Johnson, R. L. Thurmond, Y. You, F. J. McMahon, R. Jin, O. Livnah, J. Tullai, F. X. Farrell, et al.
Shared and Unique Determinants of the Erythropoietin (EPO) Receptor Are Important for Binding EPO and EPO Mimetic Peptide
J. Biol. Chem.,
May 14, 1999;
274(20):
14163 - 14169.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
O. Livnah, E. A. Stura, S. A. Middleton, D. L. Johnson, L. K. Jolliffe, and I. A. Wilson
Crystallographic Evidence for Preformed Dimers of Erythropoietin Receptor Before Ligand Activation
Science,
February 12, 1999;
283(5404):
987 - 990.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
V. C. Broudy, N. L. Lin, H.-J. Buhring, N. Komatsu, and T. J. Kavanagh
Analysis of c-kit Receptor Dimerization by Fluorescence Resonance Energy Transfer
Blood,
February 1, 1998;
91(3):
898 - 906.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Liu, K. Shen, Z. Liu, and C. T. Noguchi
Regulated Human Erythropoietin Receptor Expression in Mouse Brain
J. Biol. Chem.,
December 19, 1997;
272(51):
32395 - 32400.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. A. Gregoli and M. C. Bondurant
The Roles of Bcl-XL and Apopain in the Control of Erythropoiesis by Erythropoietin
Blood,
July 15, 1997;
90(2):
630 - 640.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. O. de Jong, Y. Westerman, G. Wagemaker, and A. W. Wognum
Coexpression of Kit and the Receptors for Erythropoietin, Interleukin 6 and GM-CSF on Hemopoietic Cells
Stem Cells,
July 1, 1997;
15(4):
275 - 285.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
M. Cazzola, F. Mercuriali, and C. Brugnara
Use of Recombinant Human Erythropoietin Outside the Setting of Uremia
Blood,
June 15, 1997;
89(12):
4248 - 4267.
[Full Text]
[PDF]
|
|
|
|
|
|
|
V. C. Broudy, N. L. Lin, D. F. Sabath, T. Papayannopoulou, and K. Kaushansky
Human Platelets Display High-Affinity Receptors for Thrombopoietin
Blood,
March 15, 1997;
89(6):
1896 - 1904.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Y. Cheung, X.-Q. Zhang, K. Bokvist, D. L. Tillotson, and B. A. Miller
Modulation of Calcium Channels in Human Erythroblasts by Erythropoietin
Blood,
January 1, 1997;
89(1):
92 - 100.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. A. Middleton, D. L. Johnson, R. Jin, F. J. McMahon, A. Collins, J. Tullai, R. H. Gruninger, L. K. Jolliffe, and L. S. Mulcahy
Identification of a Critical Ligand Binding Determinant of the Human Erythropoietin Receptor. EVIDENCE FOR COMMON LIGAND BINDING MOTIFS IN THE CYTOKINE RECEPTOR FAMILY
J. Biol. Chem.,
June 14, 1996;
271(24):
14045 - 14054.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Ogilvie, X. Yu, V. Nicolas-Metral, S. M. Pulido, C. Liu, U. T. Ruegg, and C. T. Noguchi
Erythropoietin Stimulates Proliferation and Interferes with Differentiation of Myoblasts
J. Biol. Chem.,
December 8, 2000;
275(50):
39754 - 39761.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
