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Stimulatory effects of granulocyte colony-stimulating factor on colony-
forming units-spleen (CFU-S) differentiation and pre-CFU-S proliferation in
mice
T Tanaka, T Suda, J Suda, T Inoue, Y Hirabayashi, H Hirai, F Takaku and Y Miura
Department of Medicine, Jichi Medical School, Tochigi-ken, Japan.
Granulocyte colony-stimulating factor (G-CSF) was reported to increase the
number of colony-forming units-spleen (CFU-S) and multilineage colonies as
well as myeloid-committed cells. We investigated the effects of G-CSF on
myeloid progenitors and primitive stem cells in a mouse bone marrow
transplantation (BMT) system. Lethally irradiated mice received BM cells
from untreated or 5-fluorouracil-treated mice, and then were administered
G-CSF or carrier buffer (control) for 5 days from immediately after BMT. A
pre-CFU-S assay was performed by the repeated transplantation of BM cells
from the first BMT recipients to other mice. By the method of polymerase
chain reaction, most of the spleen colonies in the secondary recipients
were confirmed to be derived from the first donors. G-CSF did not increase
the peripheral white blood cell count significantly, but did increase the
number of immature myeloid cells and granulocyte-macrophage colony-forming
cells in the BM. The number of erythroid cells in the BM was initially
suppressed and then increased by G-CSF treatment. In addition, the pre-
CFU-S assay showed an increase in pre-CFU-S cells due to G-CSF
administration. The number of spleen colonies of first BMT recipients did
not increase, but a higher percentage of them were committed to a certain
lineage by G-CSF treatment. These findings suggest that G-CSF has important
roles in the early stages of hematopoiesis.
Volume 77,
Issue 12,
pp. 2597-2602,
06/15/1991
Copyright © 1991 by The American Society of Hematology
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