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Support of early B-cell differentiation in mouse fetal liver by stromal
cells and interleukin-7
Y Gunji, T Sudo, J Suda, Y Yamaguchi, H Nakauchi, S Nishikawa, N Yanai, M Obinata, M Yanagisawa and Y Miura
Department of Pediatrics, Jichi Medical School, Tochigi-ken, Japan.
We compared the development of B-cell progenitors with that of myeloid
progenitors in fetal liver cells at various gestational ages. Day 12 to 14
fetal liver cells did not form pre-B-cell colonies. Pre-B-cell colonies
were developed from day 15 fetal liver cells. The incidence of colonies
increased with increases in gestational age and reached a maximum on days
18 to 19. In contrast, the incidence of myeloid colonies formed in the
presence of interleukin-3 (IL-3) and erythropoietin did not change
significantly during days 13 to 21 of gestation. After coculturing day 13
fetal liver cells with IL-7- producing stromal cell line ST-2, they could
respond to IL-7 and proliferate. Analysis of the phenotypes showed that day
13 fetal liver cells were B220-, IgM-, while culturing day 13 fetal liver
cells with ST-2 and untreated day 18 fetal liver cells contained the
population of B220+ cells. Even in the presence of IL-7-defective stromal
cell line FLS-3, IL-7-responsive cells could be induced from day 13 fetal
liver cells. IL-7 acted on B220+ cells and induced pre-B-cell colonies that
contained IgM+ cells in the methylcellulose culture. IL-7 mRNA was
expressed in days 13 and 18 fetal liver cells but not in pre-B cells or
adult liver cells. From these findings, it is suggested that stromal cells
or stromal-derived factors but not IL-7 were required for the
differentiation from B220- cells to B220+ cells. In the second stage,
B220+, IgM- cells proliferated and some of them differentiated to IgM+
cells in the presence of IL-7 alone. The two-step model can apply to in
vivo early B lymphopoiesis.
Volume 77,
Issue 12,
pp. 2612-2617,
06/15/1991
Copyright © 1991 by The American Society of Hematology
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