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Expression of c-jun during macrophage differentiation of HL-60 cells
R Gaynor, K Simon and P Koeffler
Division of Hematology-Oncology, UCLA School of Medicine, Wadsworth
Veterans Hospital.
Cellular transcription factors are important in the regulation of cellular
genes. Recent studies have indicated that a class of cellular genes known
as early response genes are important in the control of cellular growth
properties. Two of these genes, c-jun and c-fos, play an important role in
the control of cellular differentiation. Because the acute myelogenous
leukemia cell line, HL-60, is capable of differentiating to either
macrophages or granulocytes, it provides a good model to understand
differential gene expression. To determine if the modulation of c-jun was
important in the differentiation of HL-60 cells to either macrophages or
granulocytes, expression of c-jun mRNA was determined by Northern analysis
at various times following treatment with a variety of differentiating
agents, including 12- tetradeconyl-phorbol 13-acetate (TPA), retinoic acid
(RA), dimethyl sulfoxide (DMSO), or 1,25 dihydroxyvitamin D3 [1,25 (OH)2
D3]. Both TPA and 1,25(OH)2D3, which induce HL-60 cells to differentiate to
macrophages, resulted in marked increases in c-jun mRNA; while RA and DMSO,
which induce HL-60 cells to differentiate to granulocytes, did not greatly
alter c-jun mRNA expression. HL-60 cell lines resistant to macrophage
differentiation after exposure to either 1,25(OH)2D3 or TPA did not result
in increases in c-jun mRNA. These results suggest that elevation of c-jun
mRNA in HL-60 cells correlated temporally with differentiation to
macrophages. Thus, c-jun may be a critical cellular transcription factor
involved in macrophage differentiation.
Volume 77,
Issue 12,
pp. 2618-2623,
06/15/1991
Copyright © 1991 by The American Society of Hematology
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