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Antibodies to platelets in patients with anti-phospholipid antibodies
HJ Out, PG de Groot, M van Vliet, GC de Gast, HK Nieuwenhuis and RH Derksen
Department of Internal Medicine, University Hospital, Utrecht, The
Netherlands.
Binding of anti-phospholipid antibodies to circulating platelets and its
consequences on platelet activation and aggregation was investigated in 11
patients with anti-phospholipid antibodies. Seven patients had mild
thrombocytopenia. Nine healthy donors served as controls. Binding to
platelets was investigated by performing enzyme- linked immunosorbent
assays (ELISAs) with phospholipids as antigen on platelet eluates. Platelet
activation was measured by flow cytofluorometry using monoclonal antibodies
to an activation-specific lysosomal membrane protein. Findings in ELISA
were compared with results of a conventional immunofluorescence method to
detect platelet autoantibodies. In seven patients antibodies to negatively
charged phospholipids were present in platelet eluates. In all
thrombocytopenic patients and controls the platelets were not activated and
aggregation was not impaired. There was a positive concordance of 50%
between the results of immunofluorescence and ELISA. No apparent relation
was found between the results of ELISA or immunofluorescence and platelet
counts. It is concluded that anti-phospholipid antibodies can bind to
circulating platelets. This binding is not associated with measurable
aggregation abnormalities nor with platelet activation characterized by
exposure of lysosomal membrane proteins. More studies are necessary to
determine the exact role of anti-phospholipid antibodies in the
pathogenesis of thrombocytopenia and thrombosis.
Volume 77,
Issue 12,
pp. 2655-2659,
06/15/1991
Copyright © 1991 by The American Society of Hematology
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