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Human immunodeficiency virus infection of megakaryocytic cells
M Sakaguchi, T Sato and JE Groopman
Division of Hematology/Oncology, Robert Mapplethorpe Laboratory for AIDS
Research, New England Deaconess Hospital, Boston, MA 02215.
The human immunodeficiency virus (HIV) is capable of infecting certain
cells of hematopoietic lineage, particularly monocyte-macrophages and T
lymphocytes. Recently, the possibility that cells of megakaryocytic lineage
are susceptible to HIV infection has been raised. We have characterized
infection of the permanent megakaryocytic cell line CMK by HIV in vitro.
CMK cells were easily infected by HIV type 2 (HIV-2), producing significant
amounts of virus in culture. Infection appeared to be mediated by the CD4
surface antigen on CMK cells. Three different strains of HIV-1 were able to
minimally infect CMK cells, suggesting there may be isolates of HIV tropic
for megakaryocytes. Infection of CMK cells led to downregulation of the CD4
surface antigen but no discernable change in expression of
megakaryocyte-associated proteins glycoprotein Ib and glycoprotein
IIb/IIIa. These observations support the likelihood that megakaryocytes are
susceptible to HIV infection, and cell lines of megakaryocytic origin may
provide a useful model to study effects of the retrovirus on megakaryocyte
function.
Volume 77,
Issue 3,
pp. 481-485,
02/01/1991
Copyright © 1991 by The American Society of Hematology
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