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Role of von Willebrand factor associated to extracellular matrices in
platelet adhesion
D Baruch, C Denis, C Marteaux, D Schoevaert, L Coulombel and D Meyer
INSERM U.143, Hopital de Bicetre, Paris, France.
The respective role of plasmatic and endothelial extracellular matrix
(ECM)-associated von Willebrand factor (vWF) in platelet adhesion was
investigated at a high shear rate using a parallel-plate perfusion chamber.
Incubation of the endothelial ECM with a monoclonal antibody (MoAb) to vWF,
which specifically blocks vWF binding to platelet GP Ib (MoAb 322),
inhibited 45% of platelet adhesion. Complete inhibition was achieved by
incubating both plasma and endothelial ECM with MoAb 322 at concentrations
that blocked only about 50% of adhesion when added separately. The effect
of ECM-associated vWF was further demonstrated when a fibroblastic ECM,
normally devoid of vWF, was coated with purified plasmatic vWF. Matrix
associated-vWF was able to significantly enhance platelet adhesion in both
the presence and the absence of plasmatic vWF. In contrast, this effect was
not seen on endothelial ECM. Binding of exogenous vWF to the ECM was
specific and dose dependent, reached the same value (500 ng/cm2) on both
fibroblastic ECM and endothelial ECM, but exhibited a threefold-lower
apparent dissociation constant (KD) on fibroblastic than on endothelial
ECM. Our studies suggest that vWF deposited by endothelial cells in the ECM
may be the most active form in platelet adhesion, whereas plasmatic vWF may
only play a secondary role.
Volume 77,
Issue 3,
pp. 519-527,
02/01/1991
Copyright © 1991 by The American Society of Hematology
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