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Developmental expression of mRNAs encoding platelet proteins in rat
megakaryocytes
MA Courtney, MH Stoler, VJ Marder and PJ Haidaris
Department of Medicine, University of Rochester School of Medicine &
Dentistry, NY.
The committed bone marrow megakaryocyte (MK) progenitor undergoes a series
of highly regulated stages of development resulting in a large
multi-nucleated platelet-producing cell. We studied the developmental
expression of the mRNA for two alpha granule proteins, fibronectin (FN) and
fibrinogen gamma chain (gamma-FIB), and a cytoskeletal protein, actin, in
MKs from marrow of Sprague-Dawley rats. By the method of in situ RNA:RNA
hybridization, we showed that mRNAs for the alpha granule proteins were
expressed most abundantly in a population of 15-microns diameter
promegakaryocytes and in cells as small as 10 microns whose identity as
immature MKs was inferred by positive staining for platelet- and
MK-specific markers. gamma-FIB and FN mRNAs were present in reduced
abundance in a small proportion of intermediate MKs; however, little or no
expression was seen in mature platelet-producing MKs. In contrast, high
levels of actin mRNA were expressed predominantly in mature, multi-
nucleated MKs, and less abundantly in the immature forms. These results
suggest that FN and gamma-FIB are transcribed early in MK development to
permit translation and packaging of the protein into alpha granules, after
which transcription ceases. On the other hand, transcription of actin
occurs continuously throughout development, with highest levels in mature
platelet-producing MKs, in which actin is needed for shape changes and
intracellular movement of organelles. Our data suggest that in situ RNA:RNA
hybridization for platelet-specific markers will provide additional
criteria by which to establish MK lineage in immature marrow progenitors.
Volume 77,
Issue 3,
pp. 560-568,
02/01/1991
Copyright © 1991 by The American Society of Hematology
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