T-cell translocation gene 1 (Ttg-1) encodes a nuclear protein normally
expressed in neural lineage cells
EA McGuire, AR Davis and SJ Korsmeyer
Department of Medicine, Howard Hughes Medical Institute, Washington
University, St Louis, MO.
We previously identified and cloned T-cell translocation gene 1 (Ttg- 1), a
putative zinc finger protein, as a result of its deregulated expression in
a T-cell acute lymphoblastic leukemia cell line (RPMI 8402) with a
t(11;14)(p15;q11). We have now characterized its genomic organization and
identified the major transcriptional start site to lie within an
initiator-like motif. Ttg-1 is normally expressed in mouse brain and not in
thymus. The mouse neuroblastoma cell line, N2a, also expresses Ttg-1.
Antibodies raised against a TrpE-Ttg-1 fusion protein precipitate an 18-Kd
nuclear protein from metabolically labeled 8402 cells. Immunofluorescence
of N2a cells shows a nuclear pattern. The two potential zinc finger domains
in Ttg-1 are highly homologous to similar regions in lin-11, mec-3, and
lsl-1. This data suggests that Ttg-1 may be involved in gene regulation.
Volume 77,
Issue 3,
pp. 599-606,
02/01/1991
Copyright © 1991 by The American Society of Hematology
