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Immunologic detection of endothelial cells in human whole blood
R Sbarbati, M de Boer, M Marzilli, M Scarlattini, G Rossi and JA van Mourik
C.N.R. Institute of Clinical Physiology, Pisa, Italy.
In this report we show that human endothelial cells (EC) can be detected in
circulating blood by means of the EC-specific monoclonal antibody (MoAb)
designated as CLB-HEC 19 and expressed quantitatively as number of cells
per milliliter of whole blood. We first developed a method that was able to
recover cultured human EC added to whole blood by Percoll density gradient
centrifugation. The final recovery of the EC was 91.6% (SE = 0.65%). The EC
were identified in the gradient subfractions by indirect immunofluorescence
with the MoAb CLB-HEC 19. This method was then applied to the separation
and characterization of EC or EC remnants from the whole arterial and
venous blood taken from two groups of patients subjected to heart
catheterization. Firstly, a preliminary blood screening of random samples
was performed in a group of eight patients (group I) using a scoring
evaluation for the presence of EC and the results were expressed as
positivity index. Secondly, the complete blood screening of a group of ten
patients (group II) was performed for the detection of immunofluorescent
cells and the results were expressed as number of EC per milliliter of
whole blood. Our results show in both group I and II a significant presence
of EC in the blood after catheterization compared with their basal values.
The minimal detectable concentration of EC was 0.06 cells/mL (SE = 0.057)
of whole blood. We consider this technique as a suitable clinical test for
the detection of EC injury in cardiovascular pathology.
Volume 77,
Issue 4,
pp. 764-769,
02/15/1991
Copyright © 1991 by The American Society of Hematology
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