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Human interleukin-9: genomic sequence, chromosomal location, and sequences
essential for its expression in human T-cell leukemia virus
(HTLV)-I-transformed human T cells
K Kelleher, K Bean, SC Clark, WY Leung, TL Yang-Feng, JW Chen, PF Lin, W Luo and YC Yang
Genetics Institute, Cambridge, MA.
We have isolated the genomic sequence of human interleukin-9 (IL-9) based
on its sequence homology with a human IL-9 cDNA isolated from human T-cell
leukemia virus (HTLV)-I-transformed T cells by expression cloning. The
entire genomic sequence has been determined and the gene consists of five
exons and four introns. The human IL-9 gene is mapped to the long arm of
human chromosome 5 at band 5q31-32, a region found to be deleted in a
number of patients with acquired 5q- abnormalities and hematologic
disorders. Several blocks of transcriptional control sequences have been
identified at the 5'-flanking region of the human IL-9 gene that may play
an important role in the control of IL-9 gene expression. The 5'-regulatory
region of the human IL-9 gene also contains sequences identified in the
5'-flanking regions of other cytokine genes mapped to the long arm of human
chromosome 5, including IL-3, IL-4, IL-5, and granulocyte-macrophage
colony-stimulating factor and other T-cell growth factor genes including
IL-2 and IL-6. The IL-9 gene is constitutively expressed in the
HTLV-I-transformed human T cells and the expression of IL-9 in these cells
can be further induced by 12-O-tetradecanoyl phorbol 13-acetate. Transient
transfection analysis using the plasmid containing the 5'-flanking region
of IL-9 gene upstream from the firefly luciferase ciferase report gene
indicated that the 0.9-kb Smal-Sacl fragment of the IL-9 gene contains
sequences required for the constitutive and activated expression of IL- 9
gene in HTLV-I-transformed cells. These results will now allow us to study
the regulatory mechanism of IL-9 gene expression in normal and leukemic
human T cells.
Volume 77,
Issue 7,
pp. 1436-1441,
04/01/1991
Copyright © 1991 by The American Society of Hematology
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