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CD66 identifies a neutrophil-specific epitope within the hematopoietic
system that is expressed by members of the carcinoembryonic antigen family
of adhesion molecules
SM Watt, G Sala-Newby, T Hoang, DJ Gilmore, F Grunert, G Nagel, SJ Murdoch, E Tchilian, ES Lennox and H Waldmann
Imperial Cancer Research Fund, London, UK.
Preliminary results from the IVth Leucocyte Culture Conference have
classified the monoclonal antibody (MoAb), YTH 71.3.2, as CD66. Two other
MoAbs, YPC 2/12.1 and CE6/2D3.1, share a common cellular specificity,
reacting with cells of the neutrophil series and colonic epithelium. The
YTH 71.3.2 and CE6/2D3.1 MoAbs both recognize a similar CD66 defined
epitope that is distinct from that identified by YPC 2/12.1. By Western
blotting, these antibodies react with different molecular species from
cells of different lineages. The antibodies identify 50- to 55-Kd, 80- to
100-Kd, and 130- to 200-Kd components present in a semi-purified
carcinoembryonic antigen (CEA) preparation from colonic adenocarcinomas and
a 90- to 130-Kd molecule from HL-60 cells. With the colonic cell line,
LS174T, YPC2/12.1 stains diffuse bands of 160 to 200 Kd and 90 to 130 Kd
with equal intensity, whereas the binding of CE6/2D3.1 and YTH 71.3.2 is
biased toward the lower molecular weight set of molecules. Remarkably, all
three antibodies recognize CEA-related molecules. Defined analyses using
HeLa cells transfected with CEA, NCA(NCA-50/90), and CGM6(NCA-95) cDNAs
show that the three MoAbs identify CEA to varying degrees. While YTH 71.3.2
and CE6/2D3.1 also bind to NCA-50/90, YPC 2/12.1 recognizes an epitope
expressed by both the NCA-50/90 and NCA-95 molecular species.
Volume 78,
Issue 1,
pp. 63-74,
07/01/1991
Copyright © 1991 by The American Society of Hematology
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