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Human burst-forming units-erythroid need direct interaction with stem cell
factor for further development
CH Dai, SB Krantz and KM Zsebo
Department of Medicine, Department of Veterans Affairs Medical Center,
Nashville, TN.
To understand the factors that regulate the early growth and development of
immature erythroid progenitor cells, the burst-forming units-erythroid
(BFU-E), it is necessary to have both highly purified target cells and a
medium free of serum. When highly purified human blood BFU-E were cultured
in a serum-free medium adequate for the growth of later erythroid
progenitors, BFU-E would not grow even with the addition of recombinant
human interleukin-3 (rIL-3), known to be essential for these cells.
However, the addition of recombinant human stem cell factor (rSCF), which
supports germ cell and pluripotential stem cell growth, stimulated BFU-E to
grow equally well in serum-free as in serum-containing medium. Limiting
dilution studies showed that rSCF acts directly on the BFU-E that do not
require accessory cells for growth. Furthermore, rSCF was necessary for
BFU-E development during the initial 7 days of culture, until these cells
reached the stage of the late progenitors, the colony-forming
units-erythroid (CFU-E). These studies indicate that early erythropoiesis
is dependent on the direct action of SCF that not only affects early stem
cells but is continually necessary for the further development of committed
erythroid progenitor cells until the CFU-E stage of maturation.
Volume 78,
Issue 10,
pp. 2493-2497,
11/15/1991
Copyright © 1991 by The American Society of Hematology
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