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Establishment of idiotype bearing B-lymphocyte clones from a patient with monoclonal gammopathy

A Osterborg, M Steinitz, N Lewin, S Bergenbrant, G Holm, AK Lefvert and H Mellstedt

Department of Oncology (Radiumhemmet), Karolinska Hospital, Stockholm, Sweden.

B-lymphocyte clones bearing an idiotypic Ig structure, which could also be found on the serum M-component, have been isolated from peripheral blood of a patient with monoclonal gammopathy (MG) using an immune- rosetting technique and limiting dilution. The majority of the cells had the surface phenotype id+/CD19+/CD20+/CD21+ or id+/CD19+/CD20+/CD21- . Some of the clones originated from spontaneous outgrowth of lymphocytes and some were derived from in vitro Epstein-Barr virus- infected lymphocytes. Three of the B-lymphocyte clones and the patient's bone marrow plasma cells appeared to have clonal identity as shown by the same Ig heavy chain gene rearrangements in Southern blot analysis using a JH probe. The study further supports the notion that the peripheral blood of patients with MG contains B lymphocytes that belong to the tumor clone.

Volume 78, Issue 10, pp. 2642-2649, 11/15/1991
Copyright © 1991 by The American Society of Hematology


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