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CD63/Pltgp40: a platelet activation antigen identical to the stage-
specific, melanoma-associated antigen ME491
DO Azorsa, JA Hyman and JE Hildreth
Department of Pharmacology and Molecular Sciences, Johns Hopkins University
School of Medicine, Baltimore, MD 21205.
CD63 is a 53-Kd lysosomal membrane glycoprotein that has been identified as
a platelet activation molecule. The current study presents evidence that
CD63 and Pltgp40, a platelet membrane glycoprotein identified in this
laboratory, are the same molecule and that CD63/Pltgp40 is identical to the
well-characterized, stage- specific melanoma-associated antigen ME491.
Identity of CD63 and Pltgp40 was demonstrated by immunoprecipitation and
sequential immunodepletion studies, which showed that the anti-Pltgp40
monoclonal antibody (MoAb) H5C6 and an anti-CD63 MoAb CLB/Gran 12
recognized the same 40- to 55-Kd platelet glycoprotein. In addition, the
anti-CD63 MoAb specifically recognized immunoaffinity-purified Pltgp40.
Amino acid sequences obtained from NH2-terminal and tryptic fragment
peptides of Pltgp40 were used to generate complementary DNA (cDNA) probes
using the polymerase chain reaction (PCR) technique. A 386-bp cDNA probe
partially encoding CD63/Pltgp40 and a full length cDNA probe were 100%
identical to the corresponding sequence of ME491. Antibodies H5C6 and
CLB/Gran12 immunoprecipitated a 30- to 60-Kd heterodisperse glycoprotein
from G361 melanoma cells, as had previously been reported for antibodies
recognizing ME491. These data, taken together with the extensive homology
recently reported between ME491, the Schistosoma mansoni membrane antigen
SM23, CD37, the tumor-associated antigen CO- 029, and the target of an
antiproliferative antibody-1, suggest that CD63 is a member of a new family
of related molecules.
Volume 78,
Issue 2,
pp. 280-284,
07/15/1991
Copyright © 1991 by The American Society of Hematology
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