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Transferrin-independent iron uptake supports B lymphocyte growth
PA Seligman, J Kovar, RB Schleicher and EW Gelfand
Department of Medicine, University of Colorado Health Sciences Center;
Denver 80262.
Raji, a malignant B-lymphocyte cell line containing Epstein-Barr virus
genomic elements, has been conditioned to proliferate optimally in
transferrin (Tf)-free medium containing a very low concentration of an iron
salt. We provide evidence that an Tf-independent iron uptake system is
physiologically important for maintaining the growth of these cells. The
data show that Raji cells take up iron from iron salts using a relatively
high-capacity, low-affinity, temperature- and calcium- dependent uptake
system. The apparent capacity of this system increases when: (1) cells are
cultured in Tf-free medium containing high concentrations of iron salt as
opposed to medium containing Tf; and (2) when the iron salt concentration
of Tf-free medium is lowered to about 1.6 mumol/L. Cellular iron uptake
also increases when a maximum number of cells are in S and G2 and M cell
phases of the cell cycle. The cells are sensitive to growth inhibition by
the addition of deferoxamine. This evidence supports the hypothesis that
certain malignant lymphocytes, under iron deplete conditions, fulfill an
iron requirement for proliferation by an adaptation such as Tf-independent
iron uptake.
Volume 78,
Issue 6,
pp. 1526-1531,
09/15/1991
Copyright © 1991 by The American Society of Hematology
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