|
|
 Previous Article | Table of Contents | Next Article 
Characterization of Ws mutant allele of rats: a 12-base deletion in
tyrosine kinase domain of c-kit gene
T Tsujimura, S Hirota, S Nomura, Y Niwa, M Yamazaki, T Tono, E Morii, HM Kim, K Kondo and Y Nishimune
Department of Pathology, Osaka University Medical School, Japan.
Homozygous mutant rats at the newly found white spotting (Ws) locus were
anemic and deficient in mast cells and melanocytes. Because the phenotype
of Ws/Ws rats resembled the phenotype of mice possessing a double-gene dose
of mutant alleles at the W locus and because the c-kit gene was mapped at
the W locus of mice, we characterized the c-kit gene of Ws/Ws rats. The
authentic sequence of the rat c-kit cDNA was determined by using a cDNA
library prepared from the hippocampus of Sprague-Dawley rats. The c-kit
cDNA of Ws/Ws and normal (+/+) control rats was obtained by reverse
transcriptase modification of the polymerase chain reaction. When compared
with the authentic sequence, a deletion of 12 bases was found in the c-kit
cDNA of Ws/Ws rats. This change was shown to be a result of the deletion of
the genomic DNA. Four amino acids encoded by the deleted 12 bases (ie,
Val-Lys-Gly-Asn) were located at two amino acids downstream from the
tyrosine autophosphorylation site in the c-kit kinase and were conserved
not only in mouse and human c-kit kinases but also in mouse and human c-fms
kinases (ie, receptors of colony-stimulating factor-1). Taken together, the
Ws/Ws rat is the first characterized mutant of the c-kit gene in an animal
species other than the mouse.
Volume 78,
Issue 8,
pp. 1942-1946,
10/15/1991
Copyright © 1991 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
T. Tsuruda, J. Kato, K. Hatakeyama, K. Kojima, M. Yano, Y. Yano, K. Nakamura, F. Nakamura-Uchiyama, Y. Matsushima, T. Imamura, et al.
Adventitial Mast Cells Contribute to Pathogenesis in the Progression of Abdominal Aortic Aneurysm
Circ. Res.,
June 6, 2008;
102(11):
1368 - 1377.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. K. Sarna
Are interstitial cells of Cajal plurifunction cells in the gut?
Am J Physiol Gastrointest Liver Physiol,
February 1, 2008;
294(2):
G372 - G390.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J Wang and M Hauer-Jensen
Neuroimmune interactions: potential target for mitigating or treating intestinal radiation injury
Br. J. Radiol.,
September 1, 2007;
80(Special_Issue_1):
S41 - S48.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Strbian, M.-L. Karjalainen-Lindsberg, P. T. Kovanen, T. Tatlisumak, and P. J. Lindsberg
Mast Cell Stabilization Reduces Hemorrhage Formation and Mortality After Administration of Thrombolytics in Experimental Ischemic Stroke
Circulation,
July 24, 2007;
116(4):
411 - 418.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Boerma, J. Wang, J. Wondergem, J. Joseph, X. Qiu, R. H. Kennedy, and M. Hauer-Jensen
Influence of Mast Cells on Structural and Functional Manifestations of Radiation-Induced Heart Disease
Cancer Res.,
April 15, 2005;
65(8):
3100 - 3107.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Nishikawa, Y. Doi, H. Watanabe, T. Tokairin, Y. Omori, M. Su, T. Yoshioka, and K. Enomoto
Transdifferentiation of Mature Rat Hepatocytes into Bile Duct-Like Cells in Vitro
Am. J. Pathol.,
April 1, 2005;
166(4):
1077 - 1088.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H.-B. Ruan, N. Zhang, and X. Gao
Identification of a Novel Point Mutation of Mouse Proto-Oncogene c-kit Through N-Ethyl-N-nitrosourea Mutagenesis
Genetics,
February 1, 2005;
169(2):
819 - 831.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. H. Kennedy, M. Hauer-Jensen, and J. Joseph
Cardiac function in hearts isolated from a rat model deficient in mast cells
Am J Physiol Heart Circ Physiol,
February 1, 2005;
288(2):
H632 - H637.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. P. Y. Lee, D. D. Mruk, A. M. Conway, and C. Y. Cheng
Zyxin, Axin, and Wiskott-Aldrich Syndrome Protein Are Adaptors That Link the Cadherin/Catenin Protein Complex to the Cytoskeleton at Adherens Junctions in the Seminiferous Epithelium of the Rat Testis
J Androl,
March 1, 2004;
25(2):
200 - 215.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. P.Y. Lee, D. Mruk, W. M. Lee, and C. Y. Cheng
Is the Cadherin/Catenin Complex a Functional Unit of Cell-Cell Actin-Based Adherens Junctions in the Rat Testis?
Biol Reprod,
February 1, 2003;
68(2):
489 - 508.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. C. Eckery, S. B. Lawrence, J. L. Juengel, P. Greenwood, K. P. McNatty, and A. E. Fidler
Gene Expression of the Tyrosine Kinase Receptor c-kit During Ovarian Development in the Brushtail Possum (Trichosurus vulpecula)
Biol Reprod,
February 1, 2002;
66(2):
346 - 353.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Katafuchi, A.-J. Li, S. Hirota, Y. Kitamura, and T. Hori
Impairment of Spatial Learning and Hippocampal Synaptic Potentiation in c-kit Mutant Rats
Learn. Mem.,
November 1, 2000;
7(6):
383 - 392.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
J. Santos, M. Benjamin, P.-C. Yang, T. Prior, and M. H. Perdue
Chronic stress impairs rat growth and jejunal epithelial barrier function: role of mast cells
Am J Physiol Gastrointest Liver Physiol,
June 1, 2000;
278(6):
G847 - G854.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P.-C. Yang, M. C. Berin, and M. H. Perdue
Enhanced Antigen Transport Across Rat Tracheal Epithelium Induced by Sensitization and Mast Cell Activation
J. Immunol.,
September 1, 1999;
163(5):
2769 - 2776.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Parichy, J. Rawls, S. Pratt, T. Whitfield, and S. Johnson
Zebrafish sparse corresponds to an orthologue of c-kit and is required for the morphogenesis of a subpopulation of melanocytes, but is not essential for hematopoiesis or primordial germ cell development
Development,
January 8, 1999;
126(15):
3425 - 3436.
[Abstract]
[PDF]
|
|
|
|
|
|
|
M. C. Berin, A. J. Kiliaan, P.-C. Yang, J. A. Groot, Y. Kitamura, and M. H. Perdue
The Influence of Mast Cells on Pathways of Transepithelial Antigen Transport in Rat Intestine
J. Immunol.,
September 1, 1998;
161(5):
2561 - 2566.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
