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Synergistic effects of interleukin-1 beta and interleukin-3 on the expansion of human hematopoietic progenitor cells in liquid cultures

M Kobayashi, M Imamura, Y Gotohda, S Maeda, H Iwasaki, K Sakurada, M Kasai, AJ Hapel and T Miyazaki

Third Department of Internal Medicine, Hokkaido University School of Medicine, Sapporo, Japan.

In the present study, we show that recombinant human interleukin-1 beta (rhIL-1 beta), which has no effect on the proliferation of human progenitor cells, has synergistic effects on the expansion of human progenitor cells induced by rhIL-3 in liquid cultures. The synergistic effects of rhIL-1 beta with rhIL-3 were observed in liquid cultures using not only fresh bone marrow mononuclear cells, but also selected populations of nonadherent cells, non-T nonadherent cells, and CD34- positive cells. Anti-granulocyte-macrophage colony-stimulating factor (anti-GM-CSF) antibody partially blocked the synergistic effects of rhIL-1 beta on the proliferation of colony-forming unit (CFU)-GM burst- forming unit-erythroid (BFU-E), and CFU-Mix in liquid cultures in the presence of rhIL-1 beta plus rhIL-3, suggesting that the synergistic effects of rhIL-1 beta plus rhIL-3 are explained in part by the secondary production of GM-CSF. Limiting dilution assays and liquid culture assays using CD34-positive cells indicate that rhIL-1 beta directly increases the numbers of colony-forming cells in liquid cultures. These results suggest that rhIL-1 beta has unique direct and indirect effects on the expansion of hematopoietic progenitor cells in liquid cultures.

Volume 78, Issue 8, pp. 1947-1953, 10/15/1991
Copyright © 1991 by The American Society of Hematology


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K. Imai, M. Kobayashi, J. Wang, Y. Ohiro, J.-i. Hamada, Y. Cho, M. Imamura, M. Musashi, T. Kondo, M. Hosokawa, et al.
Selective Transendothelial Migration of Hematopoietic Progenitor Cells: A Role in Homing of Progenitor Cells
Blood, January 1, 1999; 93(1): 149 - 156.
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  Copyright © 1991 by American Society of Hematology         Online ISSN: 1528-0020