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Interleukin-6 supports human megakaryocytic proliferation and
differentiation in vitro
T Imai, K Koike, T Kubo, T Kikuchi, Y Amano, M Takagi, N Okumura and T Nakahata
Department of Pediatrics, Shinshu University School of Medicine, Matsumoto,
Japan.
The effect of interleukin-6 (IL-6) on cells of human megakaryocyte (MK)
lineage from cord blood was explored. In semisolid colony assays containing
human plasma, a greater number of both MK colonies and cells per colony was
seen in the presence of IL-6 and IL-3 than in the presence of IL-3 alone.
This stimulatory effect of IL-6, observed on both small and large MK
colonies, was completely eliminated by the addition of anti-IL-6 antibody
to the culture. IL-6 alone had no effect on MK colony formation. In the
primary culture, MKs showed larger cell size and DNA content in the
presence of both IL-3 and IL-6 than IL-3 alone. The replating experiments
using immature MKs grown in the presence of IL-3 showed that IL-6
significantly augmented both cell size and DNA content. This effect was
also neutralized by an anti-IL-6 antibody. IL-3 had no tangible effect on
MK differentiation. Synergism between IL-6 and IL-3 on MK differentiation
was not confirmed. These results suggest that IL-6 is a synergistic factor
in the proliferation of MK progenitors and a direct effector of
differentiation of immature MKs on in vitro human megakaryocytopoiesis.
Volume 78,
Issue 8,
pp. 1969-1974,
10/15/1991
Copyright © 1991 by The American Society of Hematology
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