Reassignment of the human CSF1 gene to chromosome 1p13-p21
SW Morris, MB Valentine, DN Shapiro, JE Sublett, LL Deaven, JT Foust, WM Roberts, DP Cerretti and AT Look
Department of Hematology-Oncology, St Jude Children's Research Hospital,
Memphis, TN 38105.
Human macrophage colony-stimulating factor (CSF-1 or M-CSF) is encoded by a
single gene that was previously assigned to the long arm of chromosome 5,
band q33.1, in a region adjacent to the gene encoding its receptor
(Pettenati MJ, et al, Proc Natl Acad Sci USA 84:2970, 1987). Using
fluorescence in situ hybridization with genomic probes to examine normal
metaphase chromosomes, we reassigned the human CSF1 gene to the short arm
of chromosome 1, bands p13-p21. We confirmed this result by hybridizing a
CSF1 cDNA probe to filters containing flow-sorted chromosomes and by
identifying CSF1 sequences in DNAs extracted from human x rodent somatic
cell hybrids that contained human chromosome 1 but not human chromosome 5.
Our findings are consistent with studies that have shown tight linkage
between the murine CSF1 and amylase genes, as part of a conserved linkage
group between mouse chromosome 3 and the short arm of human chromosome 1,
which also includes the genes encoding the beta subunits of thyrotropin and
nerve growth factor. Assignment of the CSF1 gene to chromosome 1 at bands
p13-p21 raises the possibility that it may be altered by certain nonrandom
chromosomal abnormalities arising in human hematopoietic malignancies and
solid tumors.
Volume 78,
Issue 8,
pp. 2013-2020,
10/15/1991
Copyright © 1991 by The American Society of Hematology
