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Interleukin-4 is an autocrine growth factor secreted by the L-428 Reed-
Sternberg cell
SR Newcom, AA Ansari and L Gu
Department of Medicine, Emory University School of Medicine, Atlanta, GA
30303.
Recent evidence indicates that Reed-Sternberg (RS) cells from many cases of
Hodgkin's disease have features of activated lymphocytes and that
lymphokines from activated lymphocytes induce proliferation of L- 428 RS
cells. It is shown here that a lymphokine similar to a lymphokine secreted
by activated lymphocytes is secreted by L-428 cells. This lymphokine has a
molecular weight approximately equal to 68,000 daltons, identical to
glycosylated recombinant interleukin-4 (rIL-4), and cross-reacts with
monoclonal anti-IL-4 in Western immunoblotting. This Hodgkin's cell growth
factor (HCGF) is 100% neutralized by polyclonal anti-IL-4 antibodies and
competes for the IL- 4 receptor. After acid-elution, the L-428 RS cell has
been shown to have 3,396 +/- 120 high-affinity receptor sites/cell. HCGF
competes with rIL-4 for this receptor and L-428 cells contain mRNA for
IL-4. Although all evidence indicates that IL-4 is an important secreted
autocrine growth factor for L-428 RS cells, anti-IL-4 has no effect on the
sustained serum-free growth of these Hodgkin's cells, suggesting that
either the IL-4 receptor and the IL-4 receptor-growth factor complex are
protected from antibody inhibition or other mechanisms are responsible for
the sustained proliferation of L-428 RS cells.
Volume 79,
Issue 1,
pp. 191-197,
01/01/1992
Copyright © 1992 by The American Society of Hematology
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