|
|
 Previous Article | Table of Contents | Next Article 
c-myc is an erythropoietin early response gene in normal erythroid cells:
evidence for a protein kinase C-mediated signal
R Spangler and AJ Sytkowski
Laboratory for Cell and Molecular Biology, New England Deaconess Hospital,
Boston, MA 02215.
The proto-oncogene c-myc has been identified as an early response gene for
erythropoietin (Epo) in transformed murine erythroleukemia cells. Epo
activation of c-myc in these cells requires protein kinase C. We now show
the fidelity of this signaling pathway in normal erythroid cells isolated
from the spleens of phenylhydrazine-treated mice. Mouse spleen cells rich
in erythroid progenitors were washed free of endogenous Epo and then
incubated in the absence of Epo. Subsequent addition of Epo for 1 hour led
to a dramatic elevation of c-myc transcript. Addition of the protein
synthesis inhibitor cycloheximide did not prevent the c-myc response, thus
identifying c-myc as an Epo early response gene in normal cells. We used
this c-myc response as a reporter for signals initiated by the Epo
receptor. Using a series of inhibitors with known specificities and
established rank-orders of potency for different kinases, we determined
that the c-myc response to Epo was blocked with the following rank order:
staurosporine much greater than H7 greater than sangivamycin greater than
H8. This sequence is identical to that obtained using transformed cells and
is diagnostic of a protein kinase C-dependent signal. Because direct
activation of protein kinase by phorbol esters does not induce terminal
differentiation of normal cells, the pathway to c-myc established by these
studies must represent one part of a signal transduction mechanism.
Volume 79,
Issue 1,
pp. 52-57,
01/01/1992
Copyright © 1992 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
J. Fang, M. Menon, W. Kapelle, O. Bogacheva, O. Bogachev, E. Houde, S. Browne, P. Sathyanarayana, and D. M. Wojchowski
EPO modulation of cell-cycle regulatory genes, and cell division, in primary bone marrow erythroblasts
Blood,
October 1, 2007;
110(7):
2361 - 2370.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Kirito, K. Nakajima, T. Watanabe, M. Uchida, M. Tanaka, K. Ozawa, and N. Komatsu
Identification of the human erythropoietin receptor region required for Stat1 and Stat3 activation
Blood,
January 1, 2002;
99(1):
102 - 110.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Chen and A. J. Sytkowski
Erythropoietin Activates Two Distinct Signaling Pathways Required for the Initiation and the Elongation of c-myc
J. Biol. Chem.,
October 12, 2001;
276(42):
38518 - 38526.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Cui, M. Coutts, J. Stahl, and A. J. Sytkowski
Novel Interaction between the Transcription Factor CHOP (GADD153) and the Ribosomal Protein FTE/S3a Modulates Erythropoiesis
J. Biol. Chem.,
March 10, 2000;
275(11):
7591 - 7596.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Coutts, K. Cui, K. L. Davis, J. C. Keutzer, and A. J. Sytkowski
Regulated Expression and Functional Role of the Transcription Factor CHOP (GADD153) in Erythroid Growth and Differentiation
Blood,
May 15, 1999;
93(10):
3369 - 3378.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Li, K. L. Davis, and A. J. Sytkowski
Protein Kinase C-epsilon Is Necessary for Erythropoietin's Up-regulation of c-myc and for Factor-dependent DNA Synthesis. EVIDENCE FOR DISCRETE SIGNALS FOR GROWTH AND DIFFERENTIATION
J. Biol. Chem.,
October 25, 1996;
271(43):
27025 - 27030.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Tauchi, G.-S. Feng, R. Shen, M. Hoatlin, G. C. Bagby Jr., D. Kabat, L. Lu, and H. E. Broxmeyer
Involvement of SH2-containing Phosphotyrosine Phosphatase Syp in Erythropoietin Receptor Signal Transduction Pathways
J. Biol. Chem.,
March 10, 1995;
270(10):
5631 - 5635.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Sasaki, H. Yasukawa, T. Shouda, T. Kitamura, I. Dikic, and A. Yoshimura
CIS3/SOCS-3 Suppresses Erythropoietin (EPO) Signaling by Binding the EPO Receptor and JAK2
J. Biol. Chem.,
September 15, 2000;
275(38):
29338 - 29347.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
