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Decreased expression of eosinophil peroxidase and major basic protein
messenger RNAs during eosinophil maturation
V Gruart, MJ Truong, J Plumas, M Zandecki, JP Kusnierz, L Prin, D Vinatier, A Capron and M Capron
Centre d'Immunologie et de Biologie Parasitaire, Unite Mixte INSERM
U167-CNRS 624, Institut Pasteur, Lille, France.
We evaluated the levels of mRNAs encoding cationic proteins in peripheral
blood eosinophils (PBE) purified from patients with eosinophilia and in
eosinophils differentiated from cord blood cells (CBC) by culture with
recombinant human interleukin-3 (rhIL-3), rhGM- CSF, and rhIL-5. Messenger
RNAs encoding eosinophil peroxidase (EPO), major basic protein (MBP),
eosinophil-derived neurotoxin (EDN), and eosinophil cationic protein (ECP)
were detected by Northern blot hybridization with the respective specific
oligonucleotide probes. In mature PBE, MBP mRNA appeared to be absent,
whereas EPO mRNA was barely detectable in only 5 of the 19 patients. In
contrast, EDN and ECP mRNAs were observed in the PBE of all patients. In
CE, EPO, and MBP, mRNAs were abundant in immature eosinophils and their
amounts decreased after differentiation toward eosinophils. ECP and EDN
mRNAs followed the same patterns, but mRNAs were less abundant at all
timepoints studied. Study of mRNA t1/2 during the time course of
differentiation indicated that changes in the stability of the different
mRNAs were not responsible for the variations observed in the steady-state
levels. Together, these results suggest that regulation of expression
differs among EPO, MBP, EDN, and ECP mRNAs during the time course of
eosinophil differentiation.
Volume 79,
Issue 10,
pp. 2592-2597,
05/15/1992
Copyright © 1992 by The American Society of Hematology
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