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Cloning and analysis of the CD18 promoter
AG Rosmarin, R Levy and DG Tenen
Department of Medicine, Miriam Hospital, Boston, MA.
CD18, the common beta chain of the leukocyte integrin adhesion proteins, is
expressed exclusively by myeloid cells and lymphocytes. During myeloid
differentiation, the increase in CD18 cell surface expression is paralleled
by increased CD18 messenger RNA levels. Nuclear run-on studies show that
CD18 expression is transcriptionally regulated during
12-O-tetradecanoylphorbol-13-acetate (TPA)-induced HL- 60 monocytic
differentiation. The CD18 transcriptional start site was defined by primer
extension and RNAse protection. The gene encoding CD18 was cloned and a
fragment that overlaps the transcriptional start site was isolated. DNA
sequence analysis of this promoter fragment identified potential AP-1
elements that may mediate the TPA transcriptional response. The CD18
promoter also contains a putative binding site for PU.1, a
leukocyte-specific transcription factor. DNA elements resembling those
found in other myeloid and integrin promoters were identified. The CD18
promoter fragment was linked to the luciferase reporter gene,
electroporated into the U937 monocytic cell line, and its expression
increased after exposure to TPA. Thus, CD18 may serve as a model for
identifying the cis elements and trans-acting factors that regulate gene
expression during myeloid differentiation.
Volume 79,
Issue 10,
pp. 2598-2604,
05/15/1992
Copyright © 1992 by The American Society of Hematology
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