|
|
 Previous Article | Table of Contents | Next Article 
Impaired in vitro growth of purified (CD34+) hematopoietic progenitors in
human immunodeficiency virus-1 seropositive thrombocytopenic individuals
G Zauli, MC Re, B Davis, L Sen, G Visani, L Gugliotta, G Furlini and M La Placa
Institute of Microbiology, University of Bologna, Italy.
In this report the role played by human immunodeficiency virus type-1
(HIV-1) in the pathogenesis of HIV-1-related thrombocytopenia was
investigated. CD34+ hematopoietic stem/progenitor cells were purified from
the bone marrow (BM) of HIV-1(+) thrombocytopenic patients, HIV- 1(+)
nonthrombocytopenic individuals, HIV-1(-) patients with immune
thrombocytopenic purpura, and HIV-1(-) normal donors. CD34+ cells from
HIV-1(+) thrombocytopenic individuals alone showed a reduced capacity to
give rise to megakaryocytic colonies (CFU-Meg) and also a progressive and
significant decline in cell number when placed in liquid culture containing
recombinant human interleukin-3 (rIL-3). This decline involved not only
megakaryocyte but also erythroid and granulocyte/macrophage progenitors.
The defects in megakaryocyte colony formation and CD34+ cell growth did not
result from a productive HIV-1 infection of CD34+ cells. Moreover, HIV-1
DNA was absent from CD34+ cells in 10 of 12 thrombocytopenic patients
examined. On the other hand, the decreased survival/proliferation of CD34+
cells in liquid culture, within the HIV-1(+) thrombocytopenic patients, was
correlated with the presence of HIV-1 p24 antigen in BM plasma. These
results demonstrate an impairment of CD34+ cells in HIV-1(+) individuals
presenting thrombocytopenia as the only hematologic manifestation.
Furthermore, these findings suggest that increased viral replication in the
BM microenvironment may cause this impairment and possibly contributes to
HIV-induced thrombocytopenia.
Volume 79,
Issue 10,
pp. 2680-2687,
05/15/1992
Copyright © 1992 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
D. R. Clark, S. Repping, N. G. Pakker, J. M. Prins, D. W. Notermans, F. W. N. M. Wit, P. Reiss, S. A. Danner, R. A. Coutinho, J. M. A. Lange, et al.
T-cell progenitor function during progressive human immunodeficiency virus-1 infection and after antiretroviral therapy
Blood,
July 1, 2000;
96(1):
242 - 249.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Isomura, M. Yoshida, H. Namba, N. Fujiwara, R. Ohuchi, F. Uno, M. Oda, Y. Seino, and M. Yamada
Suppressive effects of human herpesvirus-6 on thrombopoietin-inducible megakaryocytic colony formation in vitro
J. Gen. Virol.,
March 1, 2000;
81(3):
663 - 673.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
J. J. Mattapallil, Z. Smit-McBride, and S. Dandekar
Gastrointestinal Epithelium Is an Early Extrathymic Site for Increased Prevalence of CD34+ Progenitor Cells in Contrast to the Thymus during Primary Simian Immunodeficiency Virus Infection
J. Virol.,
May 1, 1999;
73(5):
4518 - 4523.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
F. Voulgaropoulou, B. Tan, M. Soares, B. Hahn, and L. Ratner
Distinct Human Immunodeficiency Virus Strains in the Bone Marrow Are Associated with the Development of Thrombocytopenia
J. Virol.,
April 1, 1999;
73(4):
3497 - 3504.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
A. Moses, J. Nelson, and G. C. Bagby Jr
The Influence of Human Immunodeficiency Virus-1 on Hematopoiesis
Blood,
March 1, 1998;
91(5):
1479 - 1495.
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. F. Weichold, D. Zella, O. Barabitskaja, J. P. Maciejewski, D. E. Dunn, E. M. Sloand, and N. S. Young
Neither Human Immunodeficiency Virus-1 (HIV-1) nor HIV-2 Infects Most-Primitive Human Hematopoietic Stem Cells as Assessed in Long-Term Bone Marrow Cultures
Blood,
February 1, 1998;
91(3):
907 - 915.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. A. Holme, F. Müller, N. O. Solum, F. Brosstad, S. S. Frøland, and P. Aukrust
Enhanced activation of platelets with abnormal release of RANTES in human immunodeficiency virus type 1 infection
FASEB J,
January 1, 1998;
12(1):
79 - 90.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
S. J. Tornquist and T. B. Crawford
Suppression of Megakaryocyte Colony Growth by Plasma From Foals Infected With Equine Infectious Anemia Virus
Blood,
September 15, 1997;
90(6):
2357 - 2363.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I. Bahner, K. Kearns, S. Coutinho, E. H. Leonard, and D. B. Kohn
Infection of Human Marrow Stroma by Human Immunodeficiency Virus-1 (HIV-1) Is Both Required and Sufficient for HIV-1-Induced Hematopoietic Suppression In Vitro: Demonstration by Gene Modification of Primary Human Stroma
Blood,
September 1, 1997;
90(5):
1787 - 1798.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
U. Junker, J. J. Moon, C. S. Kalfoglou, I. Sniecinski, S. J. Forman, J. A. Zaia, H. Kaneshima, and E. Bohnlein
Hematopoietic Potential and Retroviral Transduction of CD34+Thy-1+ Peripheral Blood Stem Cells From Asymptomatic Human Immunodeficiency Virus Type-1-Infected Individuals Mobilized With Granulocyte Colony-Stimulating Factor
Blood,
June 15, 1997;
89(12):
4299 - 4306.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
