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A soluble form of Fc gamma RIII is present in human serum and other body
fluids and is elevated at sites of inflammation
HB Fleit, CD Kobasiuk, C Daly, R Furie, PC Levy and RO Webster
Department of Pathology, State University of New York, Stony Brook
11794-8691.
We have developed a highly sensitive and specific sandwich enzyme- linked
immunosorbent assay (ELISA) to measure the concentration of Fc gamma RIII
in serum and other body fluids. This ELISA is based on the use of
monoclonal antibody (MoAb) (3G8) to Fc gamma RIII and a rabbit antiserum
against Fc gamma RIII. The lower limit of detection of this ELISA was 1.5
nmol/L. The concentration of soluble Fc gamma RIII in normal serum ranged
from 7.3 to 75.9 nmol/L. Soluble Fc gamma RIII was also present in other
normal biologic fluids such as saliva, urine, and seminal fluid, but at
much lower concentrations than that found in serum. Rabbit anti-Fc gamma
RIII immunoblotted polypeptides immunoprecipitated with MoAb 3G8. Fc gamma
RIII immunoprecipitated from a neutrophil lysate migrated from 40 to 76 Kd,
whereas Fc gamma RIII immunoprecipitated from serum from the same donor
migrated from 40 to 66 Kd. The soluble form of Fc gamma RIII apparently was
bound to serum IgG, because immunoprecipitation of soluble Fc gamma RIII by
MoAb 3G8 coprecipitated polypeptides that were identified by goat antihuman
IgG. Incubation of neutrophils in vitro at 4 degrees C and 37 degrees C
showed that Fc gamma RIII was released after 30 minutes of incubation at 37
degrees C. To determine whether there was a correlation between the
concentration of soluble Fc gamma RIII in biologic fluids and inflammatory
diseases, we measured the concentration of Fc gamma RIII in the
bronchoalveolar lavage fluid from patients with adult respiratory distress
syndrome (ARDS) and in the synovial fluid from patients with various forms
of arthritis. In ARDS, we found concentrations of soluble Fc gamma RIII
that were five to seven times higher than that found in the bronchoalveolar
lavage fluids from healthy adults. The concentration of soluble Fc gamma
RIII in the synovial fluid from patients with rheumatoid arthritis ranged
from 10 nmol/L to 28 mumol/L. These results suggest that activated
neutrophils, such as those at sites of inflammation, may release Fc gamma
RIII.
Volume 79,
Issue 10,
pp. 2721-2728,
05/15/1992
Copyright © 1992 by The American Society of Hematology
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