Release of platelet-activating factor is important for the respiratory
burst induced in human eosinophils by opsonized particles
AT Tool, L Koenderman, PT Kok, M Blom, D Roos and AJ Verhoeven
Central Laboratory, The Netherlands Red Cross Blood Transfusion Service,
Amsterdam.
The respiratory burst induced in human eosinophils by serum-treated zymosan
(STZ) was found to be almost completely prevented by preincubation of the
cells with WEB 2086, an antagonist of platelet- activating factor (PAF).
When eosinophils were primed by the addition of 1 mumol/L PAF, subsequent
addition of WEB 2086 had only a minor effect on the STZ-induced respiratory
burst. These results suggest a role for PAF synthesis and PAF release in
the activation of the respiratory burst by STZ. Indeed, supernatant of
STZ-stimulated eosinophils was able to prime fresh eosinophils (as did PAF
itself), and this effect was again inhibited by WEB 2086. This indicates
that eosinophils synthesize and release PAF during STZ stimulation.
Measurements of total PAF and PAF release showed that most of the PAF
synthesized by eosinophils was released in the extracellular medium. This
study shows that synthesis and release of PAF is important for respiratory
burst activity induced in human eosinophils by STZ.
Volume 79,
Issue 10,
pp. 2729-2732,
05/15/1992
Copyright © 1992 by The American Society of Hematology
