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Direct contact between human primitive hematopoietic progenitors and bone
marrow stroma is not required for long-term in vitro hematopoiesis
CM Verfaillie
Department of Medicine, University of Minnesota, Minneapolis.
Long-term bone marrow cultures support both differentiation and
conservation of primitive human hematopoietic progenitors in the absence of
exogenous cytokines. It is believed that hematopoiesis in such cultures
requires direct contact between hematopoietic progenitors and stroma. In
the present study, we demonstrate that primitive progenitors physically
separated from the stromal layer by a 0.45- microns microporous membrane
continue to generate differentiated progenitors for at least 8 weeks.
Moreover, primitive progenitors are conserved to a greater extent under
these conditions, as when cultured in direct contact with the stroma.
However, excessive production of granulocyte-macrophage progenitors occurs
when primitive progenitors are not allowed to interact directly with the
stroma. Thus, direct contact between hematopoietic and stromal cells is not
required for either differentiation or conservation of primitive
hematopoietic progenitors but is essential for the regulated production of
mature blood elements. These findings can now be used to define the role of
diffusible factors and cell-cell or cell-extracellular matrix adhesion
events in the regulation of conservation, proliferation, and
differentiation of primitive human hematopoietic progenitors in vitro.
Volume 79,
Issue 11,
pp. 2821-2826,
06/01/1992
Copyright © 1992 by The American Society of Hematology
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